FlyBase Allele Report: Dmel\PIP5K59B[18]

General Information

Symbol

Dmel\PIP5K59B¹⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0302650

Feature type

allele

Associated gene

Dmel\PIP5K59B

Associated Insertion(s)

TI{TI}PIP5K59B¹⁸

Carried in Construct

Also Known As

dPIP5K¹⁸

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chakrabarti et al., 2015)

Mutagen

ends-out gene targeting

(Chakrabarti et al., 2015)

Progenitor genotype

Associated Insertion(s)

TI{TI}PIP5K59B¹⁸

Cytology

Description

Disruption of the kinase domain by insertion of a loxP cassette (containing w^+mW.hs) which is flanked by multiple stop codons.

(Chakrabarti et al., 2015)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage

(Kumari et al., 2022)

abnormal neurophysiology | somatic clone

(Chakrabarti et al., 2015)

decreased size | adult stage, with PIP5K59B^UAS.L, Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

partially lethal - majority die

(Chakrabarti et al., 2015)

visible | adult stage, with PIP5K59B^UAS.L, Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

Phenotype Manifest In

eye, with PIP5K59B^UAS.L, Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

eye photoreceptor cell | somatic clone

(Chakrabarti et al., 2015)

retina

(Kumari et al., 2022)

Detailed Description

Statement

Reference

PIP5K59B¹⁸ adults show decreased retinal electrical response to green light (decreased amplitude and absent transients), compared to controls.

The co-expression of PIP5K59B¹⁸ and PIP5K59B^UAS.L under the control of Scer\GAL4^ninaE.PT induces eye defects (including small size).

(Kumari et al., 2022)

Compared to wild type, photoreceptors from eyes made homozygous for PIP5K59B¹⁸ produce a much smaller receptor potential in response to light with very slow activation kinetics and response termination. PIP5K59B¹⁸ photoreceptors do not show any on or off transients.

PIP5K59B¹⁸ photoreceptors show no major defects in ultrastructure.

(Chakrabarti et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

PIP5K59B¹⁸ has abnormal neurophysiology | adult stage phenotype, non-suppressible by sktl^UAS.cRa/Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

Enhancer of

Statement

Reference

PIP5K59B¹⁸ is an enhancer of abnormal neurophysiology phenotype of rdgB⁹

(Chakrabarti et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

PIP5K59B¹⁸ has retina phenotype, non-suppressible by sktl^UAS.cRa/Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

Enhancer of

Statement

Reference

PIP5K59B¹⁸ is an enhancer of primordial germ cell | embryonic stage phenotype of WASp³/WASp¹

(Kim et al., 2021)

PIP5K59B¹⁸ is an enhancer of eye photoreceptor cell phenotype of rdgB⁹

(Chakrabarti et al., 2015)

PIP5K59B¹⁸ is an enhancer of retina | conditional phenotype of rdgB⁹

(Chakrabarti et al., 2015)

NOT Suppressor of

Statement

Reference

PIP5K59B¹⁸ is a non-suppressor of retina | conditional phenotype of norpA^P24

(Chakrabarti et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

PIP5K59B¹⁸ exacerbates the reduced electrical response to light and retinal degeneration phenotypes of rdgB⁹.

Light-dependent retinal degeneration seen in norpA³⁶ mutants is not suppressed in norpA³⁶ PIP5K59B¹⁸ animals.

(Chakrabarti et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PIP5K59B¹⁸ is rescued by PIP5K59B^+t57.178

(Kumari et al., 2022)

PIP5K59B¹⁸ is rescued by PIP5K59B^UAS.L/Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

PIP5K59B¹⁸ is rescued by PIP5K59B^+t57.178

(Chakrabarti et al., 2015)

Not rescued by

PIP5K59B¹⁸ is not rescued by PIP5K59B^K189A.UAS.L/Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

PIP5K59B¹⁸ is not rescued by PIP5K59B^UAS.S.Tag:HA/Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

Comments

PIP5K59B^+t57.178 largely corrects the abnormal light response seen in PIP5K59B¹⁸ eyes.

(Chakrabarti et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PIP5K59B¹⁸

dPIP5K¹⁸

(Kumari et al., 2022, Balakrishnan et al., 2018, Liu et al., 2018, Chakrabarti et al., 2015)

dpip5k¹⁸

(Kim et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close