FlyBase Allele Report: Dmel\park[SE.UAS]

General Information

Symbol

Dmel\park^SE.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0302844

Feature type

allele

Associated gene

Dmel\park

Associated Insertion(s)

Carried in Construct

P{UAS-park.SE}

Also Known As

UAS-dParkin S94E

Key Links

Transgenic product class

missense_variant

(Shiba-Fukushima et al., 2014)

polypeptide_post_translational_processing_variant

(Shiba-Fukushima et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Shiba-Fukushima et al., 2014)

polypeptide_post_translational_processing_variant

(Shiba-Fukushima et al., 2014)

Mutagen

in vitro construct

(Shiba-Fukushima et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-park.SE}

Cytology

Description

UASt regulatory sequences drive expression of a phosphomimetic form of park in which the serine at amino acid 94 has been replaced by glutamic acid.

(Shiba-Fukushima et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

park

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 6

CEC

(Liu et al., 2022)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dopaminergic neuron, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

indirect flight muscle cell, with Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

mitochondrion, with Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

mitochondrion, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

wing, with Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

Detailed Description

Statement

Reference

Expression of park^SE.Scer\UAS in indirect flight muscles under the control of Scer\GAL4^Mhc.PU results in over-fragmentation of mitochondria compared with controls. ATP content is reduced compared with controls in 40 day old flies.

Expression of park^SE.Scer\UAS under the control of Scer\GAL4^Mhc.PU enhances the decline in climbing ability seen in older flies. The flies have an abnormal wing posture even in young adult flies.

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^ple.PU causes a reduction in the number of tubular mitochondria in the cell bodies of dopaminergic neurons in 5 day old flies compared to controls, resulting in a single large aggregate of mitochondria in each cell body. The mitochondria seen outside of the cell body are not seen.

Flies expressing park^SE.Scer\UAS under the control of Scer\GAL4^ple.PU exhibit reduced flying ability compared to controls.

Expression of park^SE.Scer\UAS under the control of Scer\GAL4^ple.PU results in loss of dopaminergic neurons in 5-day old flies.

(Shiba-Fukushima et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, park^SE.UAS has visible phenotype, suppressible | partially by Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^ple.PU, park^SE.UAS has abnormal neuroanatomy phenotype, non-suppressible by Pink1^B9

(Shiba-Fukushima et al., 2014)

Suppressor of

Statement

Reference

park^SE.UAS/Scer\GAL4^Mhc.PU is a suppressor of short lived phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

NOT Suppressor of

Statement

Reference

park^SE.UAS/Scer\GAL4^Mhc.PU is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, park^SE.UAS has wing phenotype, suppressible | partially by Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, park^SE.UAS has indirect flight muscle cell phenotype, non-suppressible by Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

Scer\GAL4^Mhc.PU, park^SE.UAS has mitochondrion phenotype, non-suppressible by Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU, park^SE.UAS has dopaminergic neuron phenotype, non-suppressible by Pink1^B9

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU, park^SE.UAS has mitochondrion phenotype, non-suppressible by Pink1^B9

(Shiba-Fukushima et al., 2014)

Suppressor of

Statement

Reference

park^SE.UAS, Scer\GAL4^Mhc.PU is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pink1^{dsRNA.Scer\UAS} does not suppress the over-fragmentation of mitochondria seen when park^SE.Scer\UAS is expressed in indirect flight muscles under the control of Scer\GAL4^Mhc.PU.

Expression of park^SE.Scer\UAS suppresses the increase in mitochondrial length seen when Pink1^{dsRNA.Scer\UAS} is expressed under control of Scer\GAL4^Mhc.PU.

Expression of park^SE.Scer\UAS under the control of Scer\GAL4^Mhc.PU in a Pink1^B9 mutant background results in reduced ATP levels compared to expression of park^SE.Scer\UAS or Pink1^B9 alone.

Expression of park^SE.Scer\UAS under the control of Scer\GAL4^Mhc.PU fails to suppress the climbing defects seen in Pink1^B9 mutant flies.

Pink1^B9 partially suppresses the abnormal wing posture seen in flies expressing park^SE.Scer\UAS under the control of Scer\GAL4^Mhc.PU.

Pink1^B9 does not suppress the mitochondria defects seen when park^SE.Scer\UAS is expressed under the control of Scer\GAL4^ple.PU.

Pink1^B9 does not suppress the dopaminergic neuron loss seen when park^SE.Scer\UAS is expressed under the control of Scer\GAL4^ple.PU.

(Shiba-Fukushima et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of park^SE.Scer\UAS under the control of Scer\GAL4^da.PU results in lethality, either in a park^Δ21/park¹ or wild type mutant background.

(Shiba-Fukushima et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

park^SE.Scer\UAS

park^SE.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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