FlyBase Allele Report: Dmel\Ofut1[R245AKI]

General Information

Symbol

Dmel\Ofut1^R245AKI

Species

D. melanogaster

Name

FlyBase ID

FBal0302850

Feature type

allele

Associated gene

Dmel\Ofut1

Associated Insertion(s)

TI{TI}Ofut1^R245AKI

Carried in Construct

Key Links

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Ishio et al., 2015)

Progenitor genotype

Associated Insertion(s)

TI{TI}Ofut1^R245AKI

Cytology

Description

Amino acid replacement: R245A.

(Ishio et al., 2015)

Amino acid replacement (R245A) in the O-fut1 GDP-fucose binding site. A single loxP site is also present due to the method used to generate the allele.

(Ishio et al., 2015)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | third instar larval stage

(Matsumoto et al., 2016)

lethal - all die before end of larval stage

(Ishio et al., 2015)

neurogenic phenotype | heat sensitive

(Ishio et al., 2015)

Phenotype Manifest In

eo neuron | third instar larval stage

(Matsumoto et al., 2016)

sensory mother cell | somatic clone | heat sensitive

(Ishio et al., 2015)

sensory mother cell | third instar larval stage | temperature conditional

(Matsumoto et al., 2016)

wing disc | third instar larval stage | temperature conditional

(Matsumoto et al., 2016)

Detailed Description

Statement

Reference

O-fut1^R245AKI homozygous third instar larvae exhibit a strong increase in the number of wing disc sensory organ precursor cells at the non-permissive 30[o]C of temperature, but not at the permissive 25[o]C of temperature, associated to a significant increase in number of supraalar neurons, which is bigger at 30[o]C than at 25[o]C, as compared to controls.

(Matsumoto et al., 2016)

O-fut1^R245AKI homozygotes survive until the third-instar larval stage.

O-fut1^R245AKI homozygotes obtained from females carrying the O-fut1^R245AKI homozygous germline show a temperature-sensitive neurogenic phenotype. At 30[o]C, O-fut1^R245AKI m/z embryos show a highly penetrant neurogenic phenotype.

O-fut1^R245AKI homozygous wing disc clones exhibit an increase in the number of sensory organ precursors at 30[o]C, but display wild-type numbers at 18 and 25[o]C.

(Ishio et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ofut1^R245AKI has increased cell number | third instar larval stage | conditional phenotype, enhanceable by rumi⁴⁴/rumi⁴⁴

(Matsumoto et al., 2016)

Other

Statement

Reference

Ofut1^R245AKI, Uxs[+]/Uxs¹ has increased cell number | third instar larval stage phenotype

(Matsumoto et al., 2016)

Ofut1^R245AKI, shams^Δ34 has increased cell number | third instar larval stage phenotype

(Matsumoto et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ofut1^R245AKI has wing disc | third instar larval stage phenotype, enhanceable by rumi⁴⁴/rumi⁴⁴

(Matsumoto et al., 2016)

Ofut1^R245AKI has eo neuron | third instar larval stage | conditional phenotype, enhanceable by rumi⁴⁴/rumi⁴⁴

(Matsumoto et al., 2016)

Ofut1^R245AKI has sensory mother cell | somatic clone | heat sensitive phenotype, enhanceable by rumi⁴⁴

(Ishio et al., 2015)

Enhancer of

Statement

Reference

Ofut1^R245AKI is an enhancer of sensory mother cell | somatic clone | heat sensitive phenotype of rumi⁴⁴

(Ishio et al., 2015)

Other

Statement

Reference

Ofut1^R245AKI, Uxs[+]/Uxs¹ has wing disc | third instar larval stage phenotype

(Matsumoto et al., 2016)

Ofut1^R245AKI, Uxs[+]/Uxs¹ has sensory mother cell | third instar larval stage phenotype

(Matsumoto et al., 2016)

Ofut1^R245AKI, Uxs[+]/Uxs¹ has eo neuron | third instar larval stage phenotype

(Matsumoto et al., 2016)

Ofut1^R245AKI, shams^Δ34 has wing disc | third instar larval stage phenotype

(Matsumoto et al., 2016)

Ofut1^R245AKI, shams^Δ34 has sensory mother cell | third instar larval stage phenotype

(Matsumoto et al., 2016)

Ofut1^R245AKI, shams^Δ34 has eo neuron | third instar larval stage phenotype

(Matsumoto et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The third instar larval wing discs of O-fut1^R245AKI/O-fut1^R245AKI, Uxs¹/+ individuals or O-fut1^R245AKI/O-fut1^R245AKI, shams^Δ34/shams^Δ34 individuals, but not O-fut1^R245AKI/O-fut1^R245AKI, Uxs¹/Uxs¹ individuals, raised at 25[o]C of temperature, exhibit increased numbers of both sensory organ precursor cells and supraalar neurons, as compared to controls.

The increased number of supraalar neurons present in the third instar larval wing discs of O-fut1^R245AKI homozygotes raised at 25[o]C of temperature is strongly enhanced by rumi⁴⁴ homozygosity.

(Matsumoto et al., 2016)

O-fut1^R245AKI and rumi⁴⁴ homozygous double mutant wing disc clones exhibit an increase in the number of sensory organ precursors at 18, 25 and 30[o]C.

(Ishio et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

O-fut1<up>R245A knock-in</up>

(Ishio et al., 2015)

O-fut1^R245A

(Matsumoto et al., 2016)

O-fut1^R245AKI

Ofut1^R245AKI

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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