FlyBase Allele Report: Dmel\GstE14[KO]

General Information

Symbol

Dmel\GstE14^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0302938

Feature type

allele

Associated gene

Dmel\GstE14

Associated Insertion(s)

TI{TI}GstE14^KO

Carried in Construct

Also Known As

nobo^KO

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Enya et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}GstE14^KO

Cytology

Description

1033bp of the GstE14 gene region has been deleted and replaced with a loxP-flanked hsp70-w cassette. This results in a 680bp deletion in the 696bp GstE14 coding sequence.

(Enya et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:13,239,712..13,240,743 [+]

Comment:

Inferred boundaries of a deletion that removes the GstE14 coding region. The deletion boundaries were inferred from primer sequences.

(Enya et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Enya et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous GstE14^KO mutants exhibit embryonic lethality, and the embryos do not produce a differentiated cuticle structure. They also display abnormal morphogenetic movements that involve the failure of head involution, defects in dorsal closure and abnormal gut looping. Treatment with 20-hydroxyecdysone (20E) midway through embryogenesis overcomes the embryonic lethality, allowing embryos to hatch into first instar larvae. Lethality is almost completely rescued when the mother's food is supplemented with either cholesterol or 7-dehydrocholesterol (7dC).

Homozygous GstE14^KO mutant larvae (obtained by maternal administration of cholesterol) exhibit a developmental arrest phenotype at the first or second instar larval stage, and none of them reach the third instar larval stage. This developmental arrest is overcome when the diet is supplemented with 20-hydroxyecdysone (20E), but not with cholesterol of 7-dehydrocholesterol (7dC).

(Enya et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

GstE14^KO has lethal - all die during embryonic stage | recessive phenotype, suppressible by Bmor\GSTe7^UAS.Tag:HA/Scer\GAL4^phtm.PO

(Enya et al., 2014)

NOT suppressed by

Statement

Reference

GstE14^KO has lethal - all die during embryonic stage | recessive phenotype, non-suppressible by Scer\GAL4^phtm.PO/GstE4^UAS.cEa

(Enya et al., 2014)

GstE14^KO has lethal - all die during embryonic stage | recessive phenotype, non-suppressible by GstE12^UAS.cEa/Scer\GAL4^phtm.PO

(Enya et al., 2014)

GstE14^KO has lethal - all die during embryonic stage | recessive phenotype, non-suppressible by Scer\GAL4^phtm.PO/se^UAS.cKa

(Enya et al., 2014)

GstE14^KO has lethal - all die during embryonic stage | recessive phenotype, non-suppressible by GstO1^UAS.cKa/Scer\GAL4^phtm.PO

(Enya et al., 2014)

GstE14^KO has lethal - all die during embryonic stage | recessive phenotype, non-suppressible by GstO2^A.UAS/Scer\GAL4^phtm.PO

(Enya et al., 2014)

GstE14^KO has lethal - all die during embryonic stage | recessive phenotype, non-suppressible by Scer\GAL4^phtm.PO/GstO2^B.UAS

(Enya et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of GstE4^Scer\UAS.cEa under the control of Scer\GAL4^phm.PO does not rescue the embryonic lethality associated with homozygous GstE14^KO mutants.

Expression of GstE12^Scer\UAS.cEa under the control of Scer\GAL4^phm.PO does not rescue the embryonic lethality associated with homozygous GstE14^KO mutants.

Expression of se^Scer\UAS.cKa under the control of Scer\GAL4^phm.PO does not rescue the embryonic lethality associated with homozygous GstE14^KO mutants.

Expression of GstO1^Scer\UAS.cKa under the control of Scer\GAL4^phm.PO does not rescue the embryonic lethality associated with homozygous GstE14^KO mutants.

Expression of GstO2^A.Scer\UAS under the control of Scer\GAL4^phm.PO does not rescue the embryonic lethality associated with homozygous GstE14^KO mutants.

Expression of GstO2^B.Scer\UAS under the control of Scer\GAL4^phm.PO does not rescue the embryonic lethality associated with homozygous GstE14^KO mutants.

(Enya et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Bmor\GSTe7^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^phm.PO suppresses the embryonic lethality associated with homozygous GstE14^KO. The flies are able to complete their development and grow to the adult stages.

(Enya et al., 2014)

Complementation and Rescue Data

Rescued by

GstE14^KO is rescued by GstE14^UAS.Tag:HA/Scer\GAL4^phtm.PO

(Enya et al., 2014)

Comments

Expression of GstE14^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^phm.PO rescues the lethality seen in GstE14^KO mutant embryos.

(Enya et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GstE14^KO

nobo^KO

(Koiwai et al., 2020, Enya et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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