FlyBase Allele Report: Dmel\RtGEF[UAS.Tag:HA]

General Information

Symbol

Dmel\RtGEF^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0303112

Feature type

allele

Associated gene

Dmel\RtGEF

Associated Insertion(s)

Carried in Construct

P{UAS-HA-RtGEF}

Key Links

Transgenic product class

wild_type

(Dent et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Dent et al., 2015)

Mutagen

in vitro construct

(Dent et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-HA-RtGEF}

Cytology

Description

UASt regulatory sequences drive expression of RtGEF that is tagged at the N-terminal end with Tag:HA.

(Dent et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

RtGEF

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-enhanceable by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-enhanceable by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

NOT suppressed by

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-suppressible by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-suppressible by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

Enhancer of

Statement

Reference

RtGEF^UAS.Tag:HA/Git^UAS.Tag:MYC, Scer\GAL4^nub.PU is an enhancer of visible phenotype of Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

NOT Enhancer of

Statement

Reference

RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU is a non-enhancer of visible phenotype of Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

Other

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU has visible phenotype

(Dent et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-enhanceable by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-enhanceable by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

NOT suppressed by

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-suppressible by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-suppressible by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

Enhancer of

Statement

Reference

RtGEF^UAS.Tag:HA/Git^UAS.Tag:MYC, Scer\GAL4^nub.PU is an enhancer of wing phenotype of Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

NOT Enhancer of

Statement

Reference

RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU is a non-enhancer of wing phenotype of Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

Other

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has eye phenotype

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU has wing phenotype

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype

(Dent et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of two copies each of RtGEF^{Scer\UAS.T:Ivir\HA1} and Git^{Scer\UAS.T:Hsap\MYC} under the control of two copies of Scer\GAL4^nub.PU results in a significant reduction in wing size compared to controls.

The reduction in wing area seen in flies expressing hpo^Scer\UAS.cUa under the control of Scer\GAL4^nub.PU is not enhanced by co-expression of either RtGEF^{Scer\UAS.T:Ivir\HA1} or Git^{Scer\UAS.T:Hsap\MYC} alone, but it is significantly enhanced by simultaneous co-expression of both RtGEF^{Scer\UAS.T:Ivir\HA1} and Git^{Scer\UAS.T:Hsap\MYC} together.

The strong reduction in wing area seen in flies co-expressing all of hpo^Scer\UAS.cUa, RtGEF^{Scer\UAS.T:Ivir\HA1} and Git^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nub.PU is unaffected if they are also carrying either Pak^KK101874 or Pak3^GD8481.

(Dent et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RtGEF^{Scer\UAS.T:Ivir\HA1}

RtGEF^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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