FlyBase Allele Report: Hsap\SNCA[UAS.EGFP]

General Information

Symbol

Hsap\SNCA^UAS.EGFP

Species

H. sapiens

Name

FlyBase ID

FBal0303970

Feature type

allele

Associated gene

Hsap\SNCA

Associated Insertion(s)

Carried in Construct

P{UAS-SNCA.EGFP}

Key Links

Transgenic product class

wild_type

(Poças et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Poças et al., 2015)

Mutagen

in vitro construct

(Poças et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-SNCA.EGFP}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\SNCA fused with EGFP.

(Poças et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\SNCA

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA with Hsap\HTT^{UAS.103Q.mCherry}

(Poças et al., 2015)

model of Parkinson's disease 1

CEA

(Bridi et al., 2021, Krzystek et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mitochondrion | larval stage, with Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Detailed Description

Statement

Reference

Flies expressing Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU develop normal eye, identical to control flies.

Flies expressing Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ninaE.PU do not show any eye degeneration phenotype.

(Poças et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\SNCA^UAS.EGFP, Scer\GAL4^nSyb.PU is an enhancer of abnormal locomotor behavior | progressive phenotype of Hsap\HTT^{UAS.103Q.mCherry}, Scer\GAL4^nSyb.PU

(Poças et al., 2015)

Hsap\SNCA^UAS.EGFP, Scer\GAL4^nSyb.PU is an enhancer of short lived phenotype of Hsap\HTT^{UAS.103Q.mCherry}, Scer\GAL4^nSyb.PU

(Poças et al., 2015)

NOT Enhancer of

Statement

Reference

Hsap\SNCA^UAS.EGFP, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Hsap\HTT^{UAS.103Q.mCherry}, Scer\GAL4^GMR.PU

(Poças et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Hsap\SNCA^UAS.EGFP, Scer\GAL4^Appl.G1a has mitochondrion | larval stage phenotype, non-suppressible by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Enhancer of

Statement

Reference

Hsap\SNCA^UAS.EGFP, Scer\GAL4^ninaE.PU is an enhancer of eye photoreceptor cell phenotype of Hsap\HTT^{UAS.103Q.mCherry}, Scer\GAL4^ninaE.PU

(Poças et al., 2015)

NOT Enhancer of

Statement

Reference

Hsap\SNCA^UAS.EGFP, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Hsap\HTT^{UAS.103Q.mCherry}, Scer\GAL4^GMR.PU

(Poças et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Flies expressing Hsap\HTT^{Scer\UAS.103Q.T:Disc\RFP-mCherry} together with Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU show striking retinal degeneration, producing a strong rough eye phenotype.

Co-expression of Hsap\HTT^{Scer\UAS.103Q.T:Disc\RFP-mCherry} and Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP} specifically in the photoreceptors R1-R6 using Scer\GAL4^ninaE.PU results in a stronger and premature degeneration phenotype compared with each single transgenic line.

Co-expression of Hsap\HTT^{Scer\UAS.103Q.T:Disc\RFP-mCherry} and Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP} in the nervous system under the control of Scer\GAL4^nSyb.PU causes severe motor dysfunction and decrease in life span. Flies co-expressing Hsap\HTT^{Scer\UAS.103Q.T:Disc\RFP-mCherry} and Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP} show stronger progressive motor dysfunction than flies expressing Hsap\HTT^{Scer\UAS.103Q.T:Disc\RFP-mCherry} alone. The decrease in life span of flies expressing Hsap\HTT^{Scer\UAS.103Q.T:Disc\RFP-mCherry} is also exacerbated by the co-expression of Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP}. Neurotoxicity, motor dysfunction and fly death start around days 8-15 and their increase occur in parallel, indicating an association between these events.

(Poças et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\SNCA^{Scer\UAS.T:Avic\GFP-EGFP}

Hsap\SNCA^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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