Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R51D04 and Hsap\RELA^AD.R65B09 in adult males significantly decreases the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R51D04 and Hsap\RELA^AD.R65B09 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS}, but not shi^1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R51D04 and Hsap\RELA^AD.R65B09 in adults during training, consolidation and testing substantially reduces aversive 2 h associative olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) does not affect attractive 2 h associative olfactory memory, compared to controls; this inhibition significantly reduces appetitive, but not aversive, 1 min associative visual memory, compared to controls, with no effect on sugar attraction; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, and inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R51D04 and Hsap\RELA^AD.R65B09 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.