Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R55D08 and Hsap\RELA^AD.R21D02 in third instar larvae significantly impairs short-term associative appetitive olfactory memory following training with fructose, with no effect on innate odor (n-amylacetate) or fructose preference, compared to controls.

Optogenetic activation (using Crei\ChR2^XXL.UAS with 470 nm (blue) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R55D08 and Hsap\RELA^AD.R21D02 in third instar larvae during training or testing significantly impairs short-term appetitive olfactory memory following training with fructose, compared to controls; activation in combination with a paired odor, or fructose exposure, does not significantly affect subsequent preference for these stimuli, compared to controls.

Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R22C12 and Hsap\RELA^AD.R21D02, does not significantly affect aversive olfactory long term memory in adults, compared to wild-type controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pairs of Scer\GAL4^DBD.R12C11 and Hsap\RELA^AD.R21D02, or Scer\GAL4^DBD.R22C12 and Hsap\RELA^AD.R21D02, in adult males does not significantly affect the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R22C12 and Hsap\RELA^AD.R21D02 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R22C12 and Hsap\RELA^AD.R21D02 in adults during training, consolidation and testing does not substantially reduce aversive or attractive 2 h associative olfactory memory, or appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls, and inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R22C12 and Hsap\RELA^AD.R21D02 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R12C11 and Hsap\RELA^AD.R21D02 in adults does not affect social or locomotor behaviors, compared to controls.