FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4DBD.R55D08
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General Information
Symbol
Scer\GAL4DBD.R55D08
Species
S. cerevisiae
Name
FlyBase ID
FBal0332123
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R55D08-GAL4.DBD}
Also Known As
R55D08
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Rubin Laboratory at Janelia Farm, 2017)
Progenitor genotype
Carried in construct
P{R55D08-GAL4.DBD}
Cytology
Description

The GMR55D08 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)
Allele components
Component
Use(s)
Regulatory region(s)
GMR55D08
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval mushroom body anterior paired lateral cell

      Comment: when combined with P{R21D02-p65.AD} (combination referred to as 'SS01671')

      (Saumweber et al., 2018)
      adult mushroom body anterior paired lateral cell

      Comment: when combined with P{R21D02-p65.AD} (combination referred to as 'SS01671')

      (Truman et al., 2023)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ25XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R55D08 and Hsap\RELAAD.R21D02 in third instar larvae significantly impairs short-term associative appetitive olfactory memory following training with fructose, with no effect on innate odor (n-amylacetate) or fructose preference, compared to controls.

      Optogenetic activation (using Crei\ChR2XXL.UAS with 470 nm (blue) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R55D08 and Hsap\RELAAD.R21D02 in third instar larvae during training or testing significantly impairs short-term appetitive olfactory memory following training with fructose, compared to controls; activation in combination with a paired odor, or fructose exposure, does not significantly affect subsequent preference for these stimuli, compared to controls.

      (Saumweber et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R55D08.T:Zzzz\Zip-
      Scer\GAL4DBD.R55D08
      Name Synonyms
      Secondary FlyBase IDs
        References (8)