Pol32NR42/Pol32R2 females have short, thin bristles and moderate etching of the abdominal tergites. Mutant males have a more severe bristle and abdominal tergite phenotype.
Pol32NR42/Pol32R2 females lay eggs with an apparently normal chorion morphology, but they do not hatch. Embryos collected in a four hour period fall into three phenotypic classes: 11% have 1 nucleus, having a polar body with a star-like structure with additional or fragmented chromosomes, 57% arrest in mitotic cycles 1 to 6 with uneven distribution of nuclei and 31% appear to be degenerated, having no detectable chromatin.
Mitotic cells in Pol32NR42/Pol32R2 larval brains show a higher frequency of spontaneous chromosome breakage compared to controls. Three hours after treatment with 2.5 Gy X rays, more than 50% of mutant cells show chromosome breaks, a considerably higher proportion than control cells.
Pol32NR42/Pol32R2 animals are hypersensitive to ethyl methanesulphonate and to N-ethyl-N-nitrosourea compared to wild type.
Pol32NR42/Pol32R2 suppresses position effect variegation at the w locus caused by In(1)wm4h, but does not affect telomere position effect (TPE) in insertion lines carrying a w[+] marker.