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General Information
Symbol
Dmel\foxoUAS.cKc
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Koyama
FlyBase ID
FBal0305269
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild type foxo.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of foxoScer\UAS.cKc under the control of Scer\GAL4phm.PO results in a 10 hour delay in the 'critical weight' ecdysone peak compared to controls in response to starvation. Body size is increased when critical weight takes place.

Expression of foxoScer\UAS.cKc under the control of Scer\GAL4phm.PO in starved larvae results in pharate adults with a increased body size compared to controls.

Expression of foxoScer\UAS.cKc under the control of Scer\GAL4C-765 and Scer\GAL4Bx-MS1096 in the wing, and Scer\GAL4ey.PU and Scer\GAL4GMR.PU in the eye results in reduced organ size compared to controls.

When foxoScer\UAS.cKc is expressed under the control of Scer\GAL4phm.PO in foxoΔ94/Df(3R)Exel8159 background most larvae do not survive to the third instar larval stage.

Expression of foxoScer\UAS.cKc in a foxoΔ94/Df(3R)Exel8159 mutant background in the oenocytes under the control of Scer\GAL4Desat1.PB, or in the corpora allata under the control of Scer\GAL4Aug21 does not affect the duration of the third instar larval stage (L3) or final body size in starved larvae. Expression in the ring gland, oenocytes and the corpora allata under the control of Scer\GAL4P0206 delays metamorphosis and increases body size at the 'critical weight' ecdysone peak compared to parental controls. Feeding ecdysone to Scer\GAL4P0206>foxoScer\UAS.cKc foxoΔ94/Df(3R)Exel8159 starved early third instar larvae eliminates this delay in metamorphosis.

Prothoracic gland size is reduced in larvae expressing foxoScer\UAS.cKc under the control of Scer\GAL4P0206.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of uspScer\UAS.cUa enhances the delay in 'critical weight' ecdysone peak in response to starvation seen when foxoScer\UAS.cKc is expressed under the control of Scer\GAL4phm.PO. The increase in larval body size at critical weight is also enhanced.

Co-expression of uspScer\UAS.cUa and foxoScer\UAS.cKc under the control of Scer\GAL4phm.PO enhances the increase in pharate adult body size seen following larval starvation compared with either transgene alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
foxoScer\UAS.cKc
foxoUAS.cKc
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Koyama
Secondary FlyBase IDs
    References (1)