FlyBase Allele Report: Dmel\esg[L4]

General Information

Symbol

Dmel\esg^L4

Species

D. melanogaster

Name

FlyBase ID

FBal0316540

Feature type

allele

Associated gene

Dmel\esg

Associated Insertion(s)

P{GawB}esg^L4

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Sanchez-Díaz et al., 2015)

Mutagen

P-element activity

(Sanchez-Díaz et al., 2015)

Progenitor genotype

Associated Insertion(s)

P{GawB}esg^L4

Cytology

Description

A P{GawB} element has been inserted 39 nucleotides downstream of the esg transcription initiation site ( 2L:15333866 - R6).

(Sanchez-Díaz et al., 2015)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:15,333,905..15,333,905 [-]

(Sanchez-Díaz et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive | dominant

(Sanchez-Díaz et al., 2015)

lethal (with esg^35Ce-3)

(Sanchez-Díaz et al., 2015)

lethal | recessive

(Sanchez-Díaz et al., 2015)

lethal - all die before end of pupal stage, with Scer\GAL4^L4, esg^HMS00025

(Sanchez-Díaz et al., 2015)

Phenotype Manifest In

abdomen

(Sanchez-Díaz et al., 2015)

Detailed Description

Statement

Reference

Heterozygous esg^L4 mutant flies exhibit increased sensitivity to nicotine. Approximately 8% of heterozygous esg^L4 flies exhibit cuticle defects such as loss of bristles in the tergites and sternites, and loss of pigmentation in the dorsal abdomen. Responses to heat and ether are normal.

Expression of esg^HMS00025 under the control of Scer\GAL4^L4 enhances the cuticle phenotypes seen in heterozygous esg^L4 mutant flies from 8% to 100%. The resulting flies are not viable.

(Sanchez-Díaz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

FBal0248878:, esg^L4 has chemical sensitive | dominant phenotype, enhanceable by Scer\GAL4^L4/mir-311^UAS.cluster/mir-313^UAS.cluster/mir-310^UAS.cluster, mir-312^UAS.cluster/mir-312^UAS.cluster

(Sanchez-Díaz et al., 2015)

esg^L4 has chemical sensitive | dominant phenotype, enhanceable by Scer\GAL4^L4/mir-311^UAS.pLUC

(Sanchez-Díaz et al., 2015)

esg^L4 has chemical sensitive | dominant phenotype, enhanceable by mir-312^L70/mir-311^L70/mir-310^L70

(Sanchez-Díaz et al., 2015)

Suppressed by

Statement

Reference

esg^L4 has chemical sensitive | dominant phenotype, suppressible by Scer\GAL4^L4/mir-14^UAS.pLUC

(Sanchez-Díaz et al., 2015)

Scer\GAL4^L4, esg^L4, mir-310^UAS.cluster has partially lethal - majority die phenotype, suppressible by Scer\GAL4^L4/esg^EU143

(Sanchez-Díaz et al., 2015)

Scer\GAL4^L4, esg^L4, mir-311^UAS.cluster has partially lethal - majority die phenotype, suppressible by Scer\GAL4^L4/esg^EU143

(Sanchez-Díaz et al., 2015)

Scer\GAL4^L4, esg^L4, mir-312^UAS.cluster has partially lethal - majority die phenotype, suppressible by Scer\GAL4^L4/esg^EU143

(Sanchez-Díaz et al., 2015)

Scer\GAL4^L4, esg^L4, mir-313^UAS.cluster has partially lethal - majority die phenotype, suppressible by Scer\GAL4^L4/esg^EU143

(Sanchez-Díaz et al., 2015)

Other

Statement

Reference

Scer\GAL4^L4, esg^L4, mir-310^UAS.cluster has partially lethal - majority die phenotype

(Sanchez-Díaz et al., 2015)

Scer\GAL4^L4, esg^L4, mir-311^UAS.cluster has partially lethal - majority die phenotype

(Sanchez-Díaz et al., 2015)

Scer\GAL4^L4, esg^L4, mir-312^UAS.cluster has partially lethal - majority die phenotype

(Sanchez-Díaz et al., 2015)

Scer\GAL4^L4, esg^L4, mir-313^UAS.cluster has partially lethal - majority die phenotype

(Sanchez-Díaz et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

FBal0248878:, esg^L4 has abdomen phenotype, enhanceable by Scer\GAL4^L4/mir-311^UAS.cluster/mir-313^UAS.cluster/mir-310^UAS.cluster, mir-312^UAS.cluster/mir-312^UAS.cluster

(Sanchez-Díaz et al., 2015)

esg^L4 has abdomen phenotype, enhanceable by Scer\GAL4^L4/mir-311^UAS.pLUC

(Sanchez-Díaz et al., 2015)

Suppressed by

Statement

Reference

esg^L4 has abdomen phenotype, suppressible by Scer\GAL4^L4/mir-14^UAS.pLUC

(Sanchez-Díaz et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of P{UAS-mir-310.cluster.DsRed} (which consists of the mir-310^{Scer\UAS.cluster}, mir-311^{Scer\UAS.cluster}, mir-312^{Scer\UAS.cluster} and mir-313^{Scer\UAS.cluster} alleles) under the control of Scer\GAL4^L4 enhances the cuticle phenotype seen in esg^L4 mutant flies, with 100% of the resulting flies having phenotype compared to 8% in esg^L4/+. Only 14% of the flies are viable compared with 100% in esg^L4/+. The increased nicotine sensitivity is also enhanced.

Expression of mir-311^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^L4 enhances the cuticle phenotype seen in esg^L4/+ mutant flies, with 23% of the resulting flies having phenotypes compared with 8% in esg^L4/+ alone. Viability is unaffected. The increase in nicotine sensitivity is also enhanced.

Expression of mir-14^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^L4 suppresses the cuticle phenotype and nicotine sensitivity seen in esg^L4 mutant flies. Viability is unaffected.

One copy each of P{GawB}mir-310^L70 (comprised of mir-310^L70, mir-311^L70 and mir-312^L70) and esg^L4 results in increased sensitivity to nicotine compared to esg^L4/+ alone.

(Sanchez-Díaz et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

esg^L4 is rescued by esg^EU143

(Sanchez-Díaz et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

esg^L4

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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