FlyBase Allele Report: Dmel\galla-1[Δ182]

General Information

Symbol

Dmel\galla-1^Δ182

Species

D. melanogaster

Name

FlyBase ID

FBal0317013

Feature type

allele

Associated gene

Dmel\galla-1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Yeom et al., 2015)

Mutagen

Delta2-3 transposase

(Yeom et al., 2015)

Progenitor genotype

P{EPgy2}galla-1^EY01427

(Yeom et al., 2015)

Cytology

Description

Imprecise excision of P{EPgy2}CG30152^EY01427 has resulted in a 968bp deletion that removes almost the entire galla-1 coding sequence

(Yeom et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:20,671,495..20,672,462 [+]

Comment:

Inferred boundaries of a 968 bp deletion resulting from the imprecise excision of P{EPgy2}galla-1^EY01427 that extends downstream from the P element insertion site and removes most of galla-1. The deletion is reported to remove the coding sequence from amino acid 38.

(Yeom et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous galla-1^Δ182 mutants show temperature-dependent lethality. While about 50% of the mutants die during development at 25[o]C, 95% animals die at 29[o]C. At 29[o]C, more than 70% of the mutants die during embryonic stages and the rest die throughout larval and pupal stages.

galla-1^Δ182 mutant embryos show defects in chromosome segregation. They exhibit patchy areas that have centrosomes but not chromosomes. galla-1^Δ182 mutant early embryos show large areas of defective divisions such as chromosome bridges and abnormal positioning of centrosomes due to incomplete segregation.

galla-1^Δ182/Df(2R)Exel6070 mutant embryos show consistent chromosome segregation defect phenotypes such as chromosome bridges, ectopic centrosomes and abnormal patterns of spindle microtubules.

(Yeom et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

galla-1^Δ182 has partially lethal - majority die phenotype, enhanceable by galla-2^Δ203/galla-2[+]

(Yeom et al., 2015)

Suppressor of

Statement

Reference

galla-1^Δ182/galla-1^Δ182 is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC}

(Yeom et al., 2015)

galla-1^Δ182/galla-1[+] is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC}

(Yeom et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

galla-1^Δ182/galla-1^Δ182 is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC}

(Yeom et al., 2015)

galla-1^Δ182/galla-1[+] is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC}

(Yeom et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of galla-1^Δ182 does not obviously suppress the small and rough eye phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PU. Homozygous galla-1^Δ182 results in significant rescue of the eye phenotype.

The partial lethality seen in galla-1^Δ182 mutants is considerably enhanced by one copy of galla-2^Δ203.

(Yeom et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

galla-1^Δ182

(Yeom et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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