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General Information
Symbol
Dmel\crbintra.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0286307
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Crbintra, UAS-crbMyc-intra, UAS-crbintramyc, UAS-crbintra
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of crb cDNA lacking the complete extracellular domain except the N terminal region including the signal peptide, and tagged with a Tag:MYC epitope.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing crbintra.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PU have a rough eye phenotype.

Expression of crbintra.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in dramatic overgrowth of the wing disc.

Expression of crbintra.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en.PU leads to enlarged adult wings.

Expression of crbintra.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PU results in a scab in place of the eye in the adult head cuticle.

Overexpression of crbintra.Scer\UAS.T:Hsap\MYC in the renal tubules, under the control of Scer\GAL4ct.PB does not affect tubule cell morphology in stage 11 to 13 embryos. Expression in stage 16 tubules results in a loss of junctional integrity where tubule cell polarity is compromised and tubules become multilayered cords of cells lacking a clear lumen.

Expression of crbintra.Scer\UAS.T:Hsap\MYC using Scer\GAL4GMR.PF causes severe external and internal eye phenotypes.

Expression of crbintra.Scer\UAS.T:Hsap\MYC using Scer\GAL4GMR.PF disrupts the regular array of ommatidial lattices and slightly reduces the number of elav-and B-positive cells.

When expression is driven in the eye by Scer\GAL4GMR.PF causes a rough eye phenotype. 97% of the photoreceptors in the pupal eye contain multiple adherens junctions of variable length. Polarity of photoreceptor is disrupted.

When crbintra.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 in the embryo, cell polarity is disrupted.

Over expression of crbintra.Scer\UAS.T:Hsap\MYC when driven by Scer\GAL4da.G32 produces embryos with an epidermis that develops as a multilayered tissue (unlike the single layered epithelium seen in wild-type embryos). The cells also show defective polarity characteristics.

Phenotype is exactly as for crbScer\UAS.cWa, when driven by the same Scer\GAL4 lines.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4GMR.PU, crbintra.UAS.Tag:MYC has eye phenotype, suppressible | partially by galla-2Δ203/galla-2[+]

Scer\GAL4GMR.PU, crbintra.UAS.Tag:MYC has eye phenotype, suppressible | partially by galla-1Δ182/galla-1[+]

Scer\GAL4en.PU, crbintra.UAS.Tag:MYC has wing phenotype, suppressible by tai[+]/taik15101

Scer\GAL4en.PU, crbintra.UAS.Tag:MYC has wing phenotype, suppressible by tai[+]/taik05809

Scer\GAL4en.PU, crbintra.UAS.Tag:MYC has wing phenotype, suppressible by tai[+]/tai61G1

NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

Expression of galla-1NIG.30152R strongly suppresses the rough eye phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PU.

Expression of galla-1NIG.30152R strongly suppresses the wing disc overproliferation phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4Bx-MS1096.

One copy of galla-1Δ182 does not obviously suppress the small and rough eye phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PU. Homozygous galla-1Δ182 results in significant rescue of the eye phenotype.

Expression of XpdHMJ21396 strongly suppresses the rough eye phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PU.

Expression of XpdHMJ21396 strongly suppresses the wing disc overproliferation phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4Bx-MS1096.

One copy of XpdSH2137 strongly suppresses the small and rough eye phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PU.

One copy of galla-2Δ203 considerably suppresses the small and rough eye phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PU.

The presence of taik15101/+, taik05809/+ or tai61G1/+ suppresses the enlarged wings of adult females expressing crbintra.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en.PU.

Co-expression of Scer\GAL4GMR.PF mitigates the external and internal eye phenotypes seen in crbintra.Scer\UAS.T:Hsap\MYC, Scer\GAL4GMR.PF flies.

The addition of stgunspecified has no effect on the phenotype of embryos expressing crbintra.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4da.G32.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The addition of crbintra.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4385.3 partially restores the loss of the zonula adherens seen in crbunspecified embryos. The embryonic cuticle phenotype is also partially restored.

Rescues crb2 as does crbScer\UAS.cWa, restoring an almost wild type cuticle pattern including denticle belts.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
Reported As
Symbol Synonym
crbScer\UAS.intra.T:Hsap\MYC
crbintra-myc
crbintra.Scer\UAS.T:Hsap\MYC
crbintra.UAS.T:Myc
crbintra.UAS.Tag:MYC
crumbsmyc-intra
Name Synonyms
Secondary FlyBase IDs
  • FBal0042950
  • FBal0246426
References (17)