FB2020_06, released Dec 22, 2020

Allele: Dmel\Hsc70-4wt.UAS.Tag:HA

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General Information
Symbol
Dmel\Hsc70-4wt.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0318416
Feature type
allele
Associated gene
Dmel\Hsc70-4
Associated Insertion(s)
Carried in Construct
PBac{UAS-HA-Hsc70-4.wt}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Uytterhoeven et al., 2015)
in vitro construct - coding region fusion
(Uytterhoeven et al., 2015)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      PBac{UAS-HA-Hsc70-4.wt}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UAS regulatory sequences drive expression of the wild-type Hsc70-4 open reading frame (derived from the RH04426 cDNA) which is tagged at the N-terminal end with Tag:HA.

      (Uytterhoeven et al., 2015)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      Hsc70-4
      Tagged with
      Tag:HA
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsc70-4wt.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU results in an increase in endosomal microautophagy at the larval neuromuscular junction compared to controls. Macroautophagy, synaptic vesicle endocytosis and proteasomal activity are not significantly different from wild type. Ultrastructurally, the neuromuscular junctions show a significant increase in the number of multivesicular bodies (MVBs) per bouton area and a significant increase in the number of intraluminal vesicles per MVB area.

      Expression of Hsc70-4wt.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU results in a significant increase in the mean excitatory junctional current (EJC) amplitude and in the quantal content at the neuromuscular junction compared to controls. The size of the readily releaseable vesicle pool is significantly increased.

      (Uytterhoeven et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsc70-4wt.Scer\UAS.T:Ivir\HA1
      Hsc70-4wt.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)