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General Information
Symbol
Dmel\Hsc70-4wt.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0318416
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of the wild-type Hsc70-4 open reading frame (derived from the RH04426 cDNA) which is tagged at the N-terminal end with Tag:HA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsc70-4wt.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU results in an increase in endosomal microautophagy at the larval neuromuscular junction compared to controls. Macroautophagy, synaptic vesicle endocytosis and proteasomal activity are not significantly different from wild type. Ultrastructurally, the neuromuscular junctions show a significant increase in the number of multivesicular bodies (MVBs) per bouton area and a significant increase in the number of intraluminal vesicles per MVB area.

Expression of Hsc70-4wt.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU results in a significant increase in the mean excitatory junctional current (EJC) amplitude and in the quantal content at the neuromuscular junction compared to controls. The size of the readily releaseable vesicle pool is significantly increased.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsc70-4wt.Scer\UAS.T:Ivir\HA1
Hsc70-4wt.UAS.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (4)