FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pka-C3UAS.cBa
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General Information
Symbol
Dmel\Pka-C3UAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Bettencourt da Cruz
FlyBase ID
FBal0318690
Feature type
allele
Associated gene
Dmel\Pka-C3
Associated Insertion(s)
Carried in Construct
P{UAS-Pka-C3.B}
Also Known As
UAS-PKA-C3
Key Links
Transgenic product class
wild_type
(Bettencourt da Cruz et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bettencourt da Cruz et al., 2008)
Mutagen
in vitro construct
(Bettencourt da Cruz et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Pka-C3.B}
Cytology
Description

UASt regulatory sequences drive expression of the Pka-C3 coding region.

(Bettencourt da Cruz et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Pka-C3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      A significantly reduced number of males expressing Pka-C3UAS.cBa under the control of Scer\GAL4VP16.natalisin attempt copulation compared to controls.

      (Cassar et al., 2018)

      Expression of Pka-C3Scer\UAS.cBa under the control of Scer\GAL4elav.PU results in significantly increased vacuolization in the brain as compared to controls.

      (Wentzell et al., 2014)

      Male (but not female, likely due to dosage compensation) flies with expression of Pka-C3Scer\UAS.cBa driven pan-neuronally by Scer\GAL4Appl.G1a/Y do not show signs of degeneration at 1 day old, but have a few mostly large vacuoles in neuropil after 30 days of aging; around 10% of these flies also have severe vacuolization in the lamina.

      (Bettencourt da Cruz et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Pka-C3UAS.cBa, Scer\GAL4Appl.G1a has neuropil | adult stage | male | progressive phenotype, enhanceable by sws[+]/sws1

      (Bettencourt da Cruz et al., 2008)
      Enhancer of
      Statement
      Reference

      Scer\GAL4Appl.G1a/Pka-C3UAS.cBa is an enhancer of neuropil | adult stage phenotype of sws1

      (Bettencourt da Cruz et al., 2008)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Pka-C3Scer\UAS.cBa driven pan-neuronally by Scer\GAL4Appl.G1a enhances neuropil vacuolization in sws1/sws1 flies.

      sws1/+ enhances degeneration (neuropil vacuolization) in aged male flies with expression of Pka-C3Scer\UAS.cBa driven pan-neuronally by Scer\GAL4Appl.G1a, and also results in vacuolization in aged females.

      (Bettencourt da Cruz et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4VP16.natalisin/Pka-C3UAS.cBa rescues Pka-C3NIG.6117R

      (Cassar et al., 2018)

      Scer\GAL4elav.PU/Pka-C3UAS.cBa rescues Pka-C3d

      (Cassar et al., 2018)

      Scer\GAL4VP16.natalisin/Pka-C3UAS.cBa rescues Pka-C3d

      (Cassar et al., 2018)
      Comments

      Expressing Pka-C3UAS.cBa under the control of either Scer\GAL4elav.PU or Scer\GAL4VP16.natalisin rescues the reduced occurrence of copulation in Pka-C3d/Pka-C3d males. Similarly, co-expressing Pka-C3UAS.cBa rescues the copulation phenotype in males expressing Pka-C3NIG.6117R under the control of Scer\GAL4VP16.natalisin.

      (Cassar et al., 2018)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Pka-C3Scer\UAS.cBa
      Pka-C3UAS.cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Bettencourt da Cruz
      Secondary FlyBase IDs
        References (3)