FlyBase Allele Report: Dmel\Tudor-SN[0614]

General Information

Symbol

Dmel\Tudor-SN⁰⁶¹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0319076

Feature type

allele

Associated gene

Dmel\Tudor-SN

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Ku et al., 2016)

Progenitor genotype

Cytology

Description

Amino acid replacement: E320K.

(Ku et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:261,723..261,723 [-]

Nucleotide change:

G261723A

Amino acid change:

E320K | Tudor-SN-PA; E320K | Tudor-SN-PB

Reported amino acid change:

E320K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ku et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | third instar larval stage (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

hyperplasia | third instar larval stage (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

increased cell number | adult stage (with Df(3L)BSC125)

(Ku et al., 2016)

increased cell number | adult stage (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

increased cell number | adult stage (with Tudor-SN¹²³⁶)

(Ku et al., 2016)

increased occurrence of cell division | male | third instar larval stage (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

male semi-fertile | progressive (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

Phenotype Manifest In

Nebenkern (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

onion stage spermatid (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

spermatid | adult stage (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

spermatocyte | increased number (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

spermatogonium | third instar larval stage (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

testis (with Df(3L)BSC125)

(Ku et al., 2016)

testis (with Tudor-SN¹¹²⁰)

(Ku et al., 2016)

testis (with Tudor-SN¹²³⁶)

(Ku et al., 2016)

Detailed Description

Statement

Reference

Tudor-SN⁰⁶¹⁴/Df(3L)BSC125 (2-day old males) have swollen apical tips of testes containing excessive numbers of early spermatogenic cells (42% show this phenotype). Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ (81%) or Tudor-SN⁰⁶¹⁴/Tudor-SN¹²³⁶ (45%) transheterozygotes show the same phenotype; ovaries appear normal in Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ females. Tudor-SN⁰⁶¹⁴/Tudor-SN¹²³⁶ males have initially normal but progressively reduced fertility as they get older (around 20% male fertility at 12-15 days old), compared to wild type.

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ show an over-proliferation of cells (likely spermatocytes) in the swollen apical tip of testes; a few bundles of mislocalized elongated spermatids with heads residing at the apical tip of the testis are also present (62% display this phenotype). Germline stem cell number is similar to wild type. Mutant third instar larval testes have a higher mitotic index of spermatogonia and are larger than wild type larval testes. In Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ mutant testes, round onion stage spermatids containing a single over-sized Nebenkern associated with two haploid nuclei are observed; 2 week old mutant adult testes have fewer spermatid bundles compared to wild type. Overall, mutant testes have higher proliferation of spermatogonia but compromised differentiation of spermatocytes.

(Ku et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ has male semi-fertile | progressive phenotype, suppressible by piwi²/piwi¹

(Ku et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ has testis phenotype, suppressible | partially by piwi[+]/piwi¹

(Ku et al., 2016)

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ has testis phenotype, suppressible | partially by piwi²/piwi¹

(Ku et al., 2016)

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ has testis phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/piwi^GD11827

(Ku et al., 2016)

NOT suppressed by

Statement

Reference

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ has testis phenotype, non-suppressible by piwi^GD11827/Scer\GAL4^NP1624

(Ku et al., 2016)

Suppressor of

Statement

Reference

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ is a suppressor | partially of ovary phenotype of piwi²/piwi¹

(Ku et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

piwi¹/+ (or even more so, piwi¹/piwi²) significantly partially suppresses the swollen apical tip of the testis phenotype seen in Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ males. piwi¹/piwi² suppresses infertility seen in Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ males. Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ partially suppresses ovary defects in piwi¹/piwi² females. Expression of piwi^GD11827 driven in the germline by Scer\GAL4^{nos.UTR.T:Hsim\VP16} (but not Scer\GAL4^tj-NP1624) suppresses the testis swollen apical tip phenotype seen in Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ males.

(Ku et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Tudor-SN^UAS.Tag:FLAG

(Ku et al., 2016)

Comments

Expression of Tudor-SN^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} partially rescues the swollen testis apical tip phenotype and reduced fertility of Tudor-SN¹¹²⁰/Tudor-SN⁰⁶¹⁴ males.

(Ku et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tudor-SN⁰⁶¹⁴

tsn⁰⁶¹⁴

(Ku et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

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