FlyBase Allele Report: Dmel\Clbn[1Q]

General Information

Symbol

Dmel\Clbn^1Q

Species

D. melanogaster

Name

FlyBase ID

FBal0319136

Feature type

allele

Associated gene

Dmel\Clbn

Associated Insertion(s)

TI{TI}Clbn^1Q

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Wang et al., 2013)

Mutagen

ends-out gene targeting

(Wang et al., 2013)

Progenitor genotype

Associated Insertion(s)

TI{TI}Clbn^1Q

Cytology

Description

The entire coding region of Clbn has been deleted and replaced with a Avic\GFP^arm.PG marker.

(Wang et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates intestinal cancer

modeled by Ras85D^V12.cUa.UAS

(Dai et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Wang et al., 2013)

decreased cell death | third instar larval stage | conditional

(Wang et al., 2013)

melanotic mass phenotype | third instar larval stage | conditional

(Wang et al., 2013)

radiation sensitive | third instar larval stage

(Wang et al., 2013)

short lived

(Wang et al., 2013)

viable

(Wang et al., 2013)

Phenotype Manifest In

melanotic mass | third instar larval stage | conditional

(Wang et al., 2013)

wing disc | third instar larval stage | conditional

(Wang et al., 2013)

Detailed Description

Statement

Reference

Clbn^1Q/Clbn^1Q flies are viable, but show small developmental delays and shortened lifespans. Mitotic chromosomes from the brains of Clbn^1Q/Clbn^1Q third instar larvae treated with radiation show significant increases in the number of breaks per nucleus compared to wild type (radiation sensitive). Clbn^1Q/Clbn^1Q third instar larvae develop significantly more melanotic masses after irradiation compared to wild type.

Wild type larval wing discs show two phases of cell death in response to irradiation: first signs of cell death are clear after 4h and plateau around 8h, with cell death again increasing from 16-24h; irradiated Clbn^1Q/Clbn^1Q discs show early induction of cell death (though significantly less than wild type) and then there is only a small increase in cell death from 8-24h. Similar to hid⁰⁵⁰¹⁴, irradiated Clbn^1Q/Clbn^1Q discs show significantly less apoptosis at 4h compared to wild type, and show a similar second phases of induction at 16-24h but at significantly lower levels.

(Wang et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Clbn^1Q has radiation sensitive | larval stage phenotype, non-enhanceable by p53^5A-1-4/p53^5A-1-4

(Wang et al., 2013)

Clbn^1Q has decreased cell death | third instar larval stage | conditional phenotype, non-enhanceable by hid⁰⁵⁰¹⁴

(Wang et al., 2013)

NOT suppressed by

Statement

Reference

Clbn^1Q has radiation sensitive | larval stage phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Wang et al., 2013)

Clbn^1Q has decreased cell death | third instar larval stage | conditional phenotype, non-suppressible by hid⁰⁵⁰¹⁴

(Wang et al., 2013)

Enhancer of

Statement

Reference

Clbn^1Q/Clbn^1Q is an enhancer of melanotic mass phenotype | third instar larval stage phenotype of tefu^stg

(Wang et al., 2013)

NOT Enhancer of

Statement

Reference

Clbn^1Q/Clbn^1Q is a non-enhancer of radiation sensitive | third instar larval stage phenotype of p53^5A-1-4

(Wang et al., 2013)

Clbn^1Q/Clbn^1Q is a non-enhancer of decreased cell death | third instar larval stage | conditional phenotype of hid⁰⁵⁰¹⁴

(Wang et al., 2013)

NOT Suppressor of

Statement

Reference

Clbn^1Q/Clbn^1Q is a non-suppressor of decreased cell death | third instar larval stage | conditional phenotype of hid⁰⁵⁰¹⁴

(Wang et al., 2013)

Clbn^1Q/Clbn^1Q is a non-suppressor of increased cell death | third instar larval stage phenotype of hid^GMR.PG

(Wang et al., 2013)

Clbn^1Q/Clbn^1Q is a non-suppressor of radiation sensitive | third instar larval stage phenotype of p53^5A-1-4

(Wang et al., 2013)

Other

Statement

Reference

Clbn^1Q, p53^5A-1-4 has decreased cell death | third instar larval stage | conditional phenotype

(Wang et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Clbn^1Q has wing disc | third instar larval stage | conditional phenotype, non-enhanceable by hid⁰⁵⁰¹⁴

(Wang et al., 2013)

NOT suppressed by

Statement

Reference

Clbn^1Q has wing disc | third instar larval stage | conditional phenotype, non-suppressible by hid⁰⁵⁰¹⁴

(Wang et al., 2013)

Enhancer of

Statement

Reference

Clbn^1Q/Clbn^1Q is an enhancer of melanotic mass | third instar larval stage phenotype of tefu^stg

(Wang et al., 2013)

NOT Enhancer of

Statement

Reference

Clbn^1Q/Clbn^1Q is a non-enhancer of wing disc | third instar larval stage | conditional phenotype of hid⁰⁵⁰¹⁴

(Wang et al., 2013)

NOT Suppressor of

Statement

Reference

Clbn^1Q/Clbn^1Q is a non-suppressor of wing disc | third instar larval stage | conditional phenotype of hid⁰⁵⁰¹⁴

(Wang et al., 2013)

Clbn^1Q/Clbn^1Q is a non-suppressor of eye disc | third instar larval stage phenotype of hid^GMR.PG

(Wang et al., 2013)

Other

Statement

Reference

Clbn^1Q, p53^5A-1-4 has wing disc | third instar larval stage | conditional phenotype

(Wang et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Clbn^1Q/Clbn^1Q p53^5A-1-4/p53^5A-1-4 double mutants show the same degree of radiation sensitivity (increase in the number of breaks per nucleus in mitotic chromosomes in third instar larval brains compared to wild type) as Clbn^1Q/Clbn^1Q or p53^5A-1-4/p53^5A-1-4 mutants alone. Clbn^1Q/Clbn^1Q p53^5A-1-4/p53^5A-1-4 double mutant third instar larval wing discs appear to have almost a complete block of cell death (apoptosis) in response to irradiation. hid⁰⁵⁰¹⁴ Clbn^1Q/Clbn^1Q double mutants show similar apoptosis induction in wing discs in response to irradiation as either mutant alone. Clbn^1Q/Clbn^1Q does not suppress the induction of apoptosis seen in hid^GMR.PG third instar eye discs. Clbn^1Q/Clbn^1Q significantly enhances melanotic tumor formation in tefu^stg/tefu^stg third instar larvae (36.3%).

(Wang et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Clbn^1Q

clbn^1Q

(Dai et al., 2020, Wang et al., 2013)

clbn^KO

(Dai et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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