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Citation
Wang, Y., Wang, Z., Joshi, B.H., Puri, R.K., Stultz, B., Yuan, Q., Bai, Y., Zhou, P., Yuan, Z., Hursh, D.A., Bi, X. (2013). The tumor suppressor Caliban regulates DNA damage-induced apoptosis through p53-dependent and -independent activity.  Oncogene 32(33): 3857--3866.
FlyBase ID
FBrf0232069
Publication Type
Research paper
Abstract

We previously identified Caliban (Clbn) as the Drosophila homolog of human Serologically defined colon cancer antigen 1 gene and demonstrated that it could function as a tumor suppressor in human non-small-cell lung cancer (NSCLC) cells, although its mode of action was unknown. Herein, we identify roles for Clbn in DNA damage response. We generate clbn knockout flies using homologous recombination and demonstrate that they have a heightened sensitivity to irradiation. We show that normal Clbn function facilitates both p53-dependent and -independent DNA damage-induced apoptosis. Clbn coordinates different apoptosis pathways, showing a two-stage upregulation following DNA damage. Clbn has proapoptotic functions, working with both caspase and the proapoptotic gene Hid. Finally, ecotopic expression of clbn(+) in NSCLC cells suppresses tumor formation in athymic nude mice. We conclude that Caliban is a regulator of DNA damage-induced apoptosis, functioning as a tumor suppressor in both p53-dependent and -independent pathways.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Oncogene
    Title
    Oncogene
    Publication Year
    1987-
    ISBN/ISSN
    0950-9232
    Data From Reference
    Alleles (9)
    Genes (6)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (5)