FlyBase Allele Report: Dmel\Sse[RNAi.147.UASp]

General Information

Symbol

Dmel\Sse^{RNAi.147.UASp}

Species

D. melanogaster

Name

FlyBase ID

FBal0319202

Feature type

allele

Associated gene

Dmel\Sse

Associated Insertion(s)

Carried in Construct

P{UASp-Sse.RNAi.147}

Key Links

Transgenic product class

RNAi_reagent

(Guo et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Guo et al., 2016)

Mutagen

in vitro construct

(Guo et al., 2016)

Progenitor genotype

Carried in construct

P{UASp-Sse.RNAi.147}

Cytology

Description

UASp regulatory sequences (from pVALIUM22) drive expression of a shRNA that targets Sse.

(Guo et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Sse

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

(Guo et al., 2016)

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

(Guo et al., 2016)

Phenotype Manifest In

polar body nucleus, with Scer\GAL4^{VP16.mat.αTub67C}

(Guo et al., 2016)

Detailed Description

Statement

Reference

Embryos with expression of Sse^{dsRNA.147.Scer\UAS.P\T} driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} arrest with a single mitotic spindle and around half of embryos lack an X-chromosome in the mitotic spindle; the majority of embryos from females contain polar bodies with 4 X-cent and 4 X-arm signals, arm cohesion persists in anaphase I and centromeric cohesion persists (96% of oocytes in meiosis II are in metaphase II, 56% of embryos with polar bodies retain centromeric cohesion). Failure of anaphase II results in generation two rather than four daughter nuclei at the completion of meiosis (both with a single X-cent signal).

(Guo et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, Sse^{RNAi.147.UASp}, vtd^GL00522 has abnormal cell cycle phenotype

(Guo et al., 2016)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, Sse^{RNAi.147.UASp}, vtd^GL00522 has polar body nucleus phenotype

(Guo et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of vtd^GL00522 leads to polar bodies containing 8 X-cent and 8 X-arm FISH signals in embryos with expression of Sse^{dsRNA.147.Scer\UAS.P\T} driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (polar body chromosomes replicate but fail to release arm cohesion).

(Guo et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sse^{RNAi.147.UASp}

Sse^RNAi147

(Guo et al., 2016)

Sse^{dsRNA.147.Scer\UAS.P\T}

Sse^{dsRNA.147.UASp}

(Wang et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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