Open Close
General Information
Symbol
Dmel\SLC22AUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Gai
FlyBase ID
FBal0319343
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of the SLC22A open reading frame.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ectopic expression of SLC22AScer\UAS.cGa in all mushroom body neurons using either the Scer\GAL4238Y or the Scer\GAL4GMR13F02 driver results in significant 3hr memory loss in an olfactory classical conditioning assay in adult flies.

Odor (Benzaldehyde and Octanol) and shock (electrical) avoidance was normal (tested with Scer\GAL4238Y and Scer\GAL4GMR13F02).

A conditional ectopic expression of SLC22AScer\UAS.cGa after eclosion in all mushroom body neurons using the Scer\GAL4238Y driver (with GAL80ts to limit the stage of expression) results in loss of 3hr memory in an olfactory classical conditioning assay whereas expression during development (before eclosion) has no effect on memory test performance in adults.

Supplementing food with dopamine or histidine impairs 3hr memory of both control adult flies and of the already memory-defective flies expressing SLC22AScer\UAS.cGa under the control of the Scer\GAL4238Y driver in an olfactory classical conditioning assay.

Supplementing food with choline impairs 3hr memory of control adult flies but improves that of the already memory-defective flies expressing SLC22AScer\UAS.cGa under the control of the Scer\GAL4238Y driver in an olfactory classical conditioning assay.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Co-expression of SLC22AScer\UAS.cGa with SLC22AKK112539 RNAi using either Scer\GAL4238Y or Scer\GAL4GMR13F02 driver fully rescues the 3hr enhanced memory phenotype in adult flies in an olfactory classical conditioning assay produced by the SLC22 knock-down.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
SLC22AScer\UAS.cGa
SLC22AUAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Gai
Secondary FlyBase IDs
    References (1)