FlyBase Allele Report: Dmel\Tm2[3.UAS.EGFP]

General Information

Symbol

Dmel\Tm2^3.UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0319427

Feature type

allele

Associated gene

Dmel\Tm2

Associated Insertion(s)

Carried in Construct

P{UAS-Tm2.GFP.3}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Williams et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-Tm2.GFP.3}

Cytology

Description

UAS regulatory sequences drive expression of a genomic fragment which which encompasses exons 3 through to the end of the coding sequence of exon 5b of Tm2. The Tm2 sequences are tagged at the C-terminal end with EGFP.

(Williams et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tm2

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a suppressor | partially of abnormal cell shape | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a suppressor | partially of myotube | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a suppressor | partially of muscle cell of lateral longitudinal muscle 1 | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a suppressor | partially of muscle cell of dorsal oblique muscle 2 | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a suppressor | partially of embryonic myoblast phenotype of hoip¹

(Williams et al., 2015)

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a suppressor of sarcomere | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

NOT Suppressor of

Statement

Reference

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a non-suppressor of muscle cell of lateral transverse muscle 1 | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a non-suppressor of muscle cell of lateral transverse muscle 2 | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4} is a non-suppressor of muscle cell of lateral transverse muscle 3 | embryonic stage phenotype of hoip¹

(Williams et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tm2^{3.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^kirre-rP298 when expressed at high levels is able to significantly partially rescue the lateral longitudinal (but not lateral transverse) muscle myotube elongation, dorsal oblique muscle 2 size, sarcomere assembly and myoblast fusion defects of hoip¹ mutant embryos, but when expressed at low levels is unable to rescue the lateral longitudinal or lateral transverse muscle myotube elongation or sarcomere assembly defects of hoip¹ mutant embryos.

(Williams et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tm2^{3.Scer\UAS.T:Avic\GFP-EGFP}

Tm2^3.UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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