UASp regulatory sequences drive expression of the open reading frame of the Bsg25D-RB isoform which is tagged at the C-terminal end with EGFP.
fertile, with Scer\GAL4elav-C155
fertile, with Scer\GAL4wor.PU
lethal - all die during P-stage, with Scer\GAL4Act5C.PI
lethal - all die during P-stage, with Scer\GAL4αTub84B.PL
viable, with Scer\GAL4elav-C155
viable, with Scer\GAL4wor.PU
Overexpression of Bsg25DScer\UAS.P\T.T:Avic\GFP-EGFP driven ubiquitously by Scer\GAL4αTub84B.PL or Scer\GAL4Act5C.PI results in early pupal lethality; flies with expression driven by Scer\GAL4elav-C155 or Scer\GAL4wor.PU are healthy and fertile.