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General Information
Symbol
Dmel\spoonmdi-1
Species
D. melanogaster
Name
FlyBase ID
FBal0319444
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
mdi1
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

2.4kb deletion that removes most of the coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

spoonmdi-1 females lay eggs with significantly reduced hatching rate and mitochondrial content when compared to controls.

spoonmdi-1/spoonmdi-1 adult females show severely reduced mtDNA replication in mitochondria in both germaria and egg chambers as well as progressive mitochondria clumping in mid- to late-stage egg chambers. The amount of mtDNA in eggs laid by spoonmdi-1/spoonmdi-1 females is highly reduced compared to wild-type, though the number of eggs produced is unchanged.

The endoplasmic reticulum structure as well as protein synthesis on the mitochondrial surface is normal in spoonmdi-1/spoonmdi-1 egg chambers.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

spoonmdi-1 females lay eggs that lack the counterselection ability of mitochondrial temperature sensitive (mt-ts, mt:CoIT300I) genome in a heteroplasmic background containing both mt-ts and wild-type mitochondrial genomes at a restrictive temperature (29[o]C) different than controls.

Xenogenetic Interactions
Statement
Reference

Ectopic expression of Hsap\AKAP1Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.PU in spoonmdi-1/spoonmdi-1 mutant background partially rescues the loss of mtDNA as well as the the low hatching rate characteristic spoonmdi-1/spoonmdi-1 eggs.

Complementation and Rescue Data
Comments

Ectopic expression of spoonScer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.PU in spoonmdi-1/spoonmdi-1 mutant background partially rescues the loss of mtDNA as well as the the low hatching rate characteristic spoonmdi-1/spoonmdi-1 eggs.

Ectopic expression of spoonΔMTS.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.PU in spoonmdi-1/spoonmdi-1 mutant background fails to rescue the loss of mtDNA as well as the the low hatching rate characteristic spoonmdi-1/spoonmdi-1 eggs.

Ectopic expression of spoonΔKH.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.PU in spoonmdi-1/spoonmdi-1 mutant background partially rescues the loss of mtDNA as well as the the low hatching rate characteristic spoonmdi-1/spoonmdi-1 eggs.

Ectopic expression of spoonΔPP1.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.PU in spoonmdi-1/spoonmdi-1 mutant background partially rescues the loss of mtDNA as well as the the low hatching rate characteristic spoonmdi-1/spoonmdi-1 eggs.

Ectopic expression of spoonΔR.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.PU in spoonmdi-1/spoonmdi-1 mutant background fails to rescue the loss of mtDNA as well as the the low hatching rate characteristic spoonmdi-1/spoonmdi-1 eggs.

Ectopic expression of spoonΔTudor.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.PU in spoonmdi-1/spoonmdi-1 mutant background fails to rescue the loss of mtDNA as well as the the low hatching rate characteristic spoonmdi-1/spoonmdi-1 eggs.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)