FlyBase Allele Report: Dmel\pall[ko]

General Information

Symbol

Dmel\pall^ko

Species

D. melanogaster

Name

FlyBase ID

FBal0319601

Feature type

allele

Associated gene

Dmel\pall

Associated Insertion(s)

TI{TI}pall^ko

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Xiao et al., 2015)

Mutagen

ends-out gene targeting

(Xiao et al., 2015)

Progenitor genotype

Associated Insertion(s)

TI{TI}pall^ko

Cytology

Description

The pall coding region has been deleted and replaced with sequence encoding a w^+* marker and the EYFP coding region.

(Xiao et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Xiao et al., 2015)

viable

(Xiao et al., 2015)

Phenotype Manifest In

embryonic/larval plasmatocyte | embryonic stage

(Xiao et al., 2015)

Detailed Description

Statement

Reference

pall^ko/pall^ko embryonic macrophages display defects in apoptotic cells clearance and have significantly lower phagocytic index compared to wild-type.

The survival rate of pall^ko/pall^ko adults upon infection with both Gram-positive and Gram-negative bacteria as well as their survival of septic injuries are comparable to that of controls. The uptake of bacteria during injections or the subsequent phagosomal degradation of bacteria are also unchanged in pall^ko/pall^ko adults compared to controls.

(Xiao et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

pall^ko is a non-enhancer of abnormal immune response | adult stage phenotype of imd^R156

(Xiao et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

pall^ko has embryonic/larval plasmatocyte | embryonic stage phenotype, suppressible by RpS6^WG1288/RpS6^WG1288

(Xiao et al., 2015)

pall^ko has embryonic/larval plasmatocyte | embryonic stage phenotype, suppressible by Rac2^UAS.cUa/Scer\GAL4^crq.Unk

(Xiao et al., 2015)

NOT suppressed by

Statement

Reference

pall^ko has embryonic/larval plasmatocyte | embryonic stage phenotype, non-suppressible by RpS6^WG1288/RpS6[+]

(Xiao et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The defects in phagocytic apoptotic cells clearance displayed by homozygous pall^ko mutant embryos can be rescued by combination with RpS6^WG1288 in homozygous, but not heterozygous state.

The defects in phagocytic apoptotic cells clearance displayed by homozygous pall^ko mutant embryos can be suppressed by expression of Rac2^Scer\UAS.cUa under the control of Scer\GAL4^crq.Unk in the mutant background.

The low survival rate upon septic injury characteristic for imd^R156 mutant adults remains unchanged in imd^R156;pall^ko double mutants.

(Xiao et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pall^ko260

(Xiao et al., 2015)

pall^ko

(Xiao et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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