FlyBase Allele Report: Dmel\Syx4[73]

General Information

Symbol

Dmel\Syx4⁷³

Species

D. melanogaster

Name

FlyBase ID

FBal0319660

Feature type

allele

Associated gene

Dmel\Syx4

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Harris et al., 2016)

amorphic allele - molecular evidence

(Harris et al., 2016)

Mutagen

Delta2-3 transposase

(Harris et al., 2016)

Progenitor genotype

P{EPgy2}Syx4^EY00005

(Harris et al., 2016)

Cytology

Description

Deletion that extends from the 5'UTR through the entire coding region of Syx4 (left breakpoint: X:2738999 , right breakpoint: X:2750535..2752545 , release 6 genome sequence). Generated by imprecise excision of P{EPgy2}Syx4^EY00005.

(Harris et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | recessive | third instar larval stage

(Harris et al., 2016)

abnormal neuroanatomy | third instar larval stage (with Df(1)ED6630)

(Harris et al., 2016)

lethal - all die during P-stage

(Harris et al., 2016)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Harris et al., 2016)

NMJ bouton | third instar larval stage

(Harris et al., 2016)

NMJ bouton | third instar larval stage (with Df(1)ED6630)

(Harris et al., 2016)

presynaptic active zone | third instar larval stage

(Harris et al., 2016)

Detailed Description

Statement

Reference

Syx4⁷³/Syx4⁷³ or Syx4⁷³/Df(1)ED6630 third instar larvae have significantly fewer synaptic boutons at the neuromuscular junction compared to controls. Syx4⁷³/Syx4⁷³ third instar larvae have reduced active zone density at presynaptic zones of NMJs, but there are no significant defects in the organization of individual release sites. Syx4⁷³/+ heterozygotes have similar numbers of boutons as controls. Syx4⁷³/Y hemizygotes die at the pupal stage. Syx4⁷³/Syx4⁷³ larvae have a significantly reduced ability to grow new "ghost" boutons under high K[+] stimulation at the neuromuscular junction compared to controls and heterozygotes.

(Harris et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Syx4⁷³ has abnormal neuroanatomy | third instar larval stage | recessive phenotype, enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Harris et al., 2016)

Syx4⁷³ has abnormal neuroanatomy | third instar larval stage | recessive phenotype, enhanceable by Df(3R)Nlg1^ex3.1/Df(3R)Nlg1^ex3.1

(Harris et al., 2016)

NOT Enhanced by

Statement

Reference

Syx4⁷³ has abnormal neuroanatomy | third instar larval stage | recessive phenotype, non-enhanceable by Syt4[+]/Syt4^BA1

(Harris et al., 2016)

Enhancer of

Statement

Reference

Syx4[+]/Syx4⁷³ is an enhancer of abnormal neuroanatomy | third instar larval stage | recessive phenotype of Syt4^BA1

(Harris et al., 2016)

NOT Enhancer of

Statement

Reference

Syx4⁷³/Syx4⁷³ is a non-enhancer of abnormal neuroanatomy | third instar larval stage | recessive phenotype of Nrx-1²⁷³

(Harris et al., 2016)

Other

Statement

Reference

Syt4[+]/Syt4^BA1, Syx4⁷³ has abnormal neuroanatomy phenotype

(Harris et al., 2016)

Syt4^BA1, Syx4⁷³ has lethal - all die during second instar larval stage phenotype

(Harris et al., 2016)

Nrx-1[+]/Nrx-1²⁷³, Syx4⁷³ has abnormal neuroanatomy | third instar larval stage phenotype

(Harris et al., 2016)

Df(3R)Nlg1^ex3.1/+, Syx4⁷³ has abnormal neuroanatomy | third instar larval stage phenotype

(Harris et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Syx4⁷³ has NMJ bouton | third instar larval stage phenotype, enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Harris et al., 2016)

Syx4⁷³ has NMJ bouton | third instar larval stage phenotype, enhanceable by Df(3R)Nlg1^ex3.1/Df(3R)Nlg1^ex3.1

(Harris et al., 2016)

NOT Enhanced by

Statement

Reference

Syx4⁷³ has NMJ bouton | third instar larval stage phenotype, non-enhanceable by Syt4[+]/Syt4^BA1

(Harris et al., 2016)

Enhancer of

Statement

Reference

Syx4[+]/Syx4⁷³ is an enhancer of NMJ bouton | third instar larval stage phenotype of Syt4^BA1

(Harris et al., 2016)

NOT Enhancer of

Statement

Reference

Syx4⁷³/Syx4⁷³ is a non-enhancer of NMJ bouton | third instar larval stage phenotype of Nrx-1²⁷³

(Harris et al., 2016)

Other

Statement

Reference

Syt4[+]/Syt4^BA1, Syx4⁷³ has NMJ bouton | third instar larval stage phenotype

(Harris et al., 2016)

Nrx-1[+]/Nrx-1²⁷³, Syx4⁷³ has NMJ bouton | third instar larval stage phenotype

(Harris et al., 2016)

Df(3R)Nlg1^ex3.1/+, Syx4⁷³ has NMJ bouton | third instar larval stage phenotype

(Harris et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Syx4⁷³/+ or Syt4^BA1/+ third instar larvae do not show significant differences in bouton number at the neuromuscular junction compared to controls, but Syx4⁷³/+;Syt4^BA1//+ double heterozygotes have significantly fewer boutons compared to controls or either single heterozygote. Syx4⁷³/Y;Syt4^BA1/Syt4^BA1 mutants die during the second instar larval stage. Syx4⁷³/+ significantly enhances the reduction in bouton number at the NMJ of Syt4^BA1/Syt4^BA1 third instar larvae. Syt4^BA1/+ does not significantly enhance the reduction in bouton number at the NMJ of Syx4⁷³/Y hemizygous third instar larvae.

Syx4⁷³/+;wit^A12/+ or Syx4⁷³/+;wit^B11/+ or Syx4⁷³/+;gbb¹/+ double heterozygotic third instar larvae do not show significant differences in bouton number at the neuromuscular junction compared to wild type or single heterozygotes.

Syx4⁷³/+;Df(3R)Nlg1^ex3.1/+ or Syx4⁷³/+;Nrx-1²⁷³/+ double heterozygotic third instar larvae have significantly fewer boutons at the neuromuscular junction compared to wild type or single heterozygotes. Syx4⁷³/Syx4⁷³ does not significantly enhance the decrease in bouton number seen in Nrx-1²⁷³/Nrx-1²⁷³ or Df(3R)Nlg1^ex3.1/Df(3R)Nlg1^ex3.1 larvae. Nrx-1²⁷³/Nrx-1²⁷³ or Df(3R)Nlg1^ex3.1/Df(3R)Nlg1^ex3.1 significantly enhances the decrease in bouton number seen in Syx4⁷³/Syx4⁷³ larvae.

Syx4⁷³/+;Syt4^BA1/+ or Syx4⁷³/+;Df(3R)Nlg1^ex3.1/+ larvae have a significantly reduced ability to grow new "ghost" boutons under high K[+] stimulation at the neuromuscular junction compared to controls and single heterozygotes.

(Harris et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Syx4⁷³ is rescued by Scer\GAL4^how-24B/Syx4^UAS.A

(Harris et al., 2016)

Syx4⁷³ is rescued by Scer\GAL4^how-24B/Syx4^UAS.B

(Harris et al., 2016)

Not rescued by

Syx4⁷³ is not rescued by Syx4^UAS.A/Scer\GAL4^elav.PLu

(Harris et al., 2016)

Syx4⁷³ is not rescued by Syx4^UAS.B/Scer\GAL4^elav.PLu

(Harris et al., 2016)

Comments

Expression of Syx4^Scer\UAS.A or Syx4^Scer\UAS.B driven by Scer\GAL4^how-24B (but not Scer\GAL4^elav.PLu) rescues bouton number at the neuromuscular junction of Syx4⁷³/Syx4⁷³ third instar larvae. Expression of Syx4^Scer\UAS.A driven by Scer\GAL4^how-24B rescues active zone density at Syx4⁷³/Syx4⁷³ third instar larval NMJs.

(Harris et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Syx4⁷³

(Harris et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

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