FlyBase Allele Report: Dmel\pcm[14]

General Information

Symbol

Dmel\pcm¹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0319758

Feature type

allele

Associated gene

Dmel\pcm

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Waldron et al., 2015)

Mutagen

P-element activity

(Waldron et al., 2015)

Progenitor genotype

P{EP}EP1526

(Waldron et al., 2015)

Caused by aberration

Df(1)pcm¹⁴

Cytology

Description

A 3,501 bp deletion extending from the progenitor insertion site towards pcm, deleting 3,068 bp into the 3' of the gene, completely removing exons 7-11 and part of exon 6. The 5' of the neighbouring non-coding RNA CR43260 is also deleted.

(Waldron et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:19,485,300..19,488,800 [-]

Comment:

Inferred boundaries of a 3,501 bp deletion resulting from the imprecise excision of P{EP}EP1526. The deletion extends towards pcm from the insertion site and removes 3,068 bp from the 3' end of pcm as well as CG34260.

(Waldron et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Waldron et al., 2015)

abnormal developmental rate | larval stage

(Towler et al., 2016)

abnormal size | third instar larval stage

(Towler et al., 2016, Waldron et al., 2015)

increased cell death | third instar larval stage

(Jones et al., 2016, Waldron et al., 2015)

increased occurrence of cell division | third instar larval stage

(Waldron et al., 2015)

lethal - all die during pupal stage

(Waldron et al., 2015)

Phenotype Manifest In

eye disc | third instar larval stage

(Waldron et al., 2015)

haltere disc | third instar larval stage

(Waldron et al., 2015)

metathoracic leg disc | third instar larval stage

(Waldron et al., 2015)

wing disc | third instar larval stage

(Jones et al., 2016, Towler et al., 2016, Waldron et al., 2015)

Detailed Description

Statement

Reference

Apoptosis is seen in the wing pouch area of homozygous third larval instar wing imaginal discs (no cell death is seen in wild-type wing discs at the same stage).

(Jones et al., 2016)

pcm¹⁴ mutant third instar larvae have significantly smaller wings discs compared to controls and are developmentally delayed.

(Towler et al., 2016)

pcm¹⁴ homozygous females and hemizygous males all die during pupation before any adult structures are formed although their growth rate and size during larval stages is unaffected. Onset of pupariation however is significantly delayed by around 32 hours compared to wild-type, the larvae continue to feed during this period and are thus significantly heavier just before pupariation than controls.

pcm¹⁴/pcm⁵ transheterozygote adults cannot emerge from their pupal cases and remains stuck. The same phenotype is seen when pcm⁵ is combined with Df(1)ED7452 deficiency, suggesting that pcm¹⁴ is genetically a null allele.

pcm¹⁴ homozygote third instar larvae have significantly smaller wing, metathoracic leg, haltere as well as eye imaginal discs compared to wild-type controls. The wing discs are also delayed both in growth and differentiation (assessed by wg pattern) and even though the entry into pupal stage is delayed in pcm¹⁴ homozygotes, their wing discs just before the onset of pupariation are still significantly smaller compared to controls.

pcm¹⁴/pcm¹⁴ third instar larval wing discs display significantly increased level of apoptosis (assessed by Caspase-3 staining). However, both the mitotic and the S-phase index (calculated as number of cells per area) are also increased compared to wild-type, suggesting the wing disc cells are dividing more to counteract the cell loss due to apoptosis.

Somatic clones of pcm¹⁴/pcm¹⁴ cells in the third instar larval wing discs are much smaller compared to their wild-type twin spot counterparts.

(Waldron et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

pcm¹⁴ has abnormal size | third instar larval stage phenotype, suppressible by Scer\GAL4^69B/Dis3l2^GD9240

(Towler et al., 2016)

pcm¹⁴ has increased cell death | third instar larval stage phenotype, suppressible | partially by Df(3L)H99/+

(Waldron et al., 2015)

NOT suppressed by

Statement

Reference

pcm¹⁴ has abnormal developmental rate | larval stage phenotype, non-suppressible by Scer\GAL4^69B/Dis3l2^GD9240

(Towler et al., 2016)

Suppressor of

Statement

Reference

pcm¹⁴/pcm¹⁴ is a suppressor of abnormal size | third instar larval stage phenotype of Diap1^UAS.cHa

(Waldron et al., 2015)

pcm¹⁴/pcm¹⁴ is a suppressor of abnormal size | third instar larval stage phenotype of Dark⁸²

(Waldron et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

pcm¹⁴ has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^69B/Dis3l2^GD9240

(Towler et al., 2016)

pcm¹⁴ has wing disc | third instar larval stage phenotype, suppressible | partially by Df(3L)H99/+

(Waldron et al., 2015)

pcm¹⁴ has wing disc | third instar larval stage phenotype, suppressible | partially by Dark⁸²/Dark⁸²

(Waldron et al., 2015)

pcm¹⁴ has wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^69B/Diap1^UAS.cHa

(Waldron et al., 2015)

Suppressor of

Statement

Reference

pcm¹⁴/pcm¹⁴ is a suppressor of wing disc | third instar larval stage phenotype of Dark⁸²

(Waldron et al., 2015)

pcm¹⁴/pcm¹⁴ is a suppressor of wing disc | third instar larval stage phenotype of Diap1^UAS.cHa, Scer\GAL4^69B

(Waldron et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced wing disc size characteristic for pcm¹⁴ mutant third instar larvae is fully rescued by expression of Dis3l2^GD9240 under the control of Scer\GAL4^69B in the mutant background but the larvae are still developmentally delayed.

(Towler et al., 2016)

The dramatic increase in apoptosis (assessed by Caspase-3 staining) in the third instar larval wing disc characteristic for pcm¹⁴ homozygote mutants is strongly suppressed by combination with Df(3L)H99 in heterozygote state, the reduced size of the disc is partially restored and so is its differentiation (the disturbed wg pattern is recovered).

The reduced size of the third larval instar imaginal wing disc characteristic for pcm¹⁴ homozygotes is strongly rescued by combination with heterozygous Df(3L)H99 deficiency, homozygous Dark⁸² allele or by expression of Diap1^Scer\UAS.cHa under the control of Scer\GAL4^69B (using tub-Gal80[ts] to limit the time of expression) in the mutant background. The resulting disc size is intermediate between that of pcm¹⁴ alone and that of Df(3L)H99/+ or Dark⁸²/Dark⁸² or Diap1^Scer\UAS.cHa-expressing discs (each of which alone gives rise to wing disc bigger than wild-type) and smaller than wild-type.

(Waldron et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pcm⁵

(Waldron et al., 2015)

Rescued by

pcm¹⁴ is rescued by pcm^UAS.cWa/Scer\GAL4^69B

(Waldron et al., 2015)

Partially rescued by

pcm¹⁴ is partially rescued by Scer\GAL4^nub-AC-62/pcm^UAS.cWa

(Waldron et al., 2015)

Not rescued by

pcm¹⁴ is not rescued by pcm^UAS.ND/Scer\GAL4^69B

(Waldron et al., 2015)

Comments

The pupal lethality of pcm¹⁴ mutants is fully rescued by expression of pcm^Scer\UAS.cWa under the control of the Scer\GAL4^69B but not that of the Scer\GAL4^nub-AC-62 driver.

The reduced size of third instar larval wing discs characteristic for pcm¹⁴ homozygote larvae is fully rescued by expression of pcm^Scer\UAS.cWa when driven by the Scer\GAL4^69B and partially rescued when controlled by the Scer\GAL4^nub-AC-62 driver.

Neither the pupal lethality of pcm¹⁴ mutants nor the reduced size of their third instar larval wing discs is rescued by expression of pcm^Scer\UAS.ND under the control of Scer\GAL4^69B.

(Waldron et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

606350

w¹¹¹⁸ pcm¹⁴/FM7i, P{ActGFP}JMR3

606351

w¹¹¹⁸ pcm¹⁴ P{neoFRT}19A/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer