FlyBase Allele Report: Dmel\robo1[Tag:HA]

General Information

Symbol

Dmel\robo1^Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0320372

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

Carried in Construct

PBac{robo1-HA.B}

Also Known As

robo1::robo1, P{robo1::HArobo1}

Key Links

Transgenic product class

wild_type

(Brown et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Brown et al., 2015)

Mutagen

in vitro construct

(Brown et al., 2015)

Progenitor genotype

Carried in construct

PBac{robo1-HA.B}

Cytology

Description

2385bp of robo1 upstream flanking sequence drive expression of wild-type robo1 coding sequence. 2192bp of robo1 downstream flanking sequence is also present. The protein is tagged directly upstream of the Ig1 domain with 4 copies of Tag:HA.

(Brown et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

robo1

Encoded product / tool

robo1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of robo1^T:Ivir\HA1 in the wild-type background has no discernible effect on the structure and morphology of the embryonic ventral nerve cord, not even when two copies of the transgene are present.

(Reichert et al., 2016)

Expression of one or two copies of robo1^T:Ivir\HA1 in embryos does not produce any obvious phenotypes in the ventral nerve cord.

(Brown et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^elav.PLu, comm^UAS.Tag:HA, robo1^Tag:HA has abnormal neuroanatomy | embryonic stage phenotype

(Daiber et al., 2021)

comm^Δe39, robo1¹, robo1^Tag:HA has abnormal neuroanatomy | embryonic stage phenotype

(Daiber et al., 2021)

Scer\GAL4^elav.PLu, robo1¹, robo1^Tag:HA, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, comm^UAS.cUa, robo1^Tag:HA has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020, Brown et al., 2015)

Phenotype Manifest In

Other

Statement

Reference

comm^Δe39, robo1¹, robo1^Tag:HA has commissure | embryonic stage phenotype

(Daiber et al., 2021)

Scer\GAL4^elav.PLu, comm^UAS.Tag:HA, robo1^Tag:HA has embryonic midline of ventral nerve cord phenotype

(Daiber et al., 2021)

comm^Δe39, robo1¹, robo1^Tag:HA has embryonic midline of ventral nerve cord phenotype

(Daiber et al., 2021)

Scer\GAL4^elav.PLu, robo1¹, robo1^Tag:HA, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1¹, robo1^Tag:HA, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, comm^UAS.cUa, robo1^Tag:HA has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, comm^UAS.cUa, robo1^Tag:HA has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, comm^UAS.cUa, robo1^Tag:HA has larval ventral nerve cord | embryonic stage phenotype

(Brown et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of comm^Scer\UAS.cUa (under the control of Scer\GAL4^elav.PLu) and robo1^T:Ivir\HA1 in embryos results in an increase in axon midline crossing and corresponding thickening of commissures in the ventral nerve cord.

(Brown et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

robo1^Tag:HA rescues robo1¹

(Loy et al., 2025)

robo1^Tag:HA rescues robo1¹

(Daiber et al., 2021)

robo1^Tag:HA rescues robo1¹

(Brown and Evans, 2020)

robo1^Tag:HA rescues robo1¹

(Reichert et al., 2016)

robo1^Tag:HA rescues robo1¹

(Brown et al., 2015)

Comments

Expression of robo1^T:Ivir\HA1 in robo1¹ mutant background fully rescues the defects in midline repulsion characteristic for robo1¹ mutants and restores the normal appearance of the axon scaffold in the embryonic ventral nerve cord.

(Reichert et al., 2016)

Expression of two copies of robo1^T:Ivir\HA1 rescues the ventral nerve cord defects, FasII-expressing axon guidance defects and pCC guidance defects of robo1¹/robo1¹ mutant embryos.

(Brown et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

robo1^T:Ivir\HA1

robo1^Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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