FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\robo1ΔIg1.Tag:HA
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General Information
Symbol
Dmel\robo1ΔIg1.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0320373
Feature type
allele
Associated gene
Dmel\robo1
Associated Insertion(s)
Carried in Construct
PBac{robo1-HA.ΔIg1}
Also Known As
robo1::robo1ΔIg1
Key Links
Transgenic product class
inframe_deletion
(Brown et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Brown et al., 2015)
Mutagen
in vitro construct
(Brown et al., 2015)
Progenitor genotype
Carried in construct
PBac{robo1-HA.ΔIg1}
Cytology
Description

2385bp of robo1 upstream flanking sequence drive expression of robo1 amino acid residues 153-1395 (lacks the Ig1 domain). 2192bp of robo1 downstream flanking sequence is also present. The protein is tagged directly upstream of the Ig2 domain with 4 copies of Tag:HA.

(Brown et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
robo1
Encoded product / tool
robo1
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of robo1ΔIg1.T:Ivir\HA1 in the wild-type background has no discernible effect on the structure and morphology of the embryonic ventral nerve cord, not even when two copies of the transgene are present.

      (Reichert et al., 2016)

      Expression of robo1ΔIg1.T:Ivir\HA1 in embryos does not produce any obvious phenotypes in the ventral nerve cord.

      (Brown et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of commScer\UAS.cUa (under the control of Scer\GAL4elav.PLu) and robo1ΔIg1.T:Ivir\HA1 in embryos results in an increase in axon midline crossing and corresponding thickening of commissures in the ventral nerve cord.

      (Brown et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      robo1ΔIg1.Tag:HA fails to rescue robo11

      (Reichert et al., 2016)

      robo1ΔIg1.Tag:HA fails to rescue robo11

      (Brown et al., 2015)
      Comments

      Expression of robo1ΔIg1.T:Ivir\HA1 in robo11 mutant background fails to rescue the defects in midline axon repulsion and ectopic midline crossings observed in the ventral nerve cord of robo11 embryos.

      (Reichert et al., 2016)

      Expression of two copies of robo1ΔIg1.T:Ivir\HA1 does not rescue the ventral nerve cord defects, FasII-expressing axon guidance defects or pCC guidance defects of robo11/robo11 mutant embryos.

      (Brown et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      robo1ΔIg1.T:Ivir\HA1
      robo1ΔIg1.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)