FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Egra\PACαUAS.cSLa
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General Information
Symbol
Egra\PACαUAS.cSLa
Species
E. gracilis
Name
FlyBase ID
FBal0321165
Feature type
allele
Associated gene
Egra\PACα
Associated Insertion(s)
Carried in Construct
P{UAS-PACα.SL}
Also Known As
UAS-PACα
Transgenic product class
wild_type
(Schroder-Lang et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Schroder-Lang et al., 2007)
Mutagen
in vitro construct
(Schroder-Lang et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-PACα.SL}
Cytology
Description

UAS regulatory sequences drive expression of Egra\PACα coding sequences.

(Schroder-Lang et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Egra\PACα
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Acute activation (10 minute blue light exposure) of Zzzz\PACαScer\UAS.cSLa expressed under the control of Scer\GAL4elav.PU significantly increases arousal thresholds (the minimal vibration required to arouse a quiescent fly) compared to controls.

      Continual activation of Zzzz\PACαScer\UAS.cSLa expressed under the control of Scer\GAL4elav.PU (exposure to blue light throughout the day, but not at night) results in chronically decreased responsiveness to mechanical stimuli during both day and night. However, sleep intensity is only significantly increased at night (after the blue light is turned off).

      (van Alphen et al., 2013)

      Expression of Zzzz\PACαScer\UAS.cSLa under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22 increases response index to blue light-induced stimulation, indicating overexpression larvae are now more attracted (or less repelled) by blue light. Application of white light also induces a repression of the negative phototactic behavior of transgenic larvae.

      Expression of Zzzz\PACαScer\UAS.cSLa under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22 does not lead to adaptation of ORNs to prolonged blue light stimulation nor prevent the animals from perceiving an additional odor stimulus.

      Transgenic larvae expressing Zzzz\PACαScer\UAS.cSLa under the control of Scer\GAL4Or13a.PC, Scer\GAL4Or30a.PC, Scer\GAL4Or33b.PC, Scer\GAL4Or35a.PC, Scer\GAL4Or45a.PC, Scer\GAL4Or45b.PC, Scer\GAL4Or59a.PF, Scer\GAL4Or63a.PC, Scer\GAL4Or67b.PC, Scer\GAL4Or74a.PC, Scer\GAL4Or85c.PC, or Scer\GAL4Or94b.PC are less repelled by blue or white light (in orientation assays) than wild-type larvae. These flies also show a stronger negative phototaxis effect with blue light rather than white light (this wasn't seen with Scer\GAL4Or33b.PC and Scer\GAL4Or45a.PC drivers).

      Transgenic larvae expressing Zzzz\PACαScer\UAS.cSLa under the control of Scer\GAL4Or13a.PC, Scer\GAL4Or30a.PC, Scer\GAL4Or33b.PC, Scer\GAL4Or35a.PC, Scer\GAL4Or45a.PC, Scer\GAL4Or45b.PC, Scer\GAL4Or59a.PF, Scer\GAL4Or63a.PC, Scer\GAL4Or67b.PC, Scer\GAL4Or74a.PC, Scer\GAL4Or85c.PC, or Scer\GAL4Or94b.PC avoid octyl acetate, as in wild-type.

      Expression of Zzzz\PACαScer\UAS.cSLa under the control of Scer\GAL4Or33b.PC or Scer\GAL4Or45a.PC results in an aversion response to blue light.

      (Bellmann et al., 2010)

      Neuronal expression of Zzzz\PACαScer\UAS.cSLa under the control of Scer\GAL4elav.PU results in adult flies without any obvious behavioral phenotype. Irradiating adult flies with blue light (but not other wavelengths) results in periods of hyperactivity and unusual freezing behavior in flies expressing Zzzz\PACαScer\UAS.cSLa but not in wild-type. In addition, grooming behavior is substantially reduced in these flies in the presence of blue light. This behavior is lost a few seconds after switching the flies back to white light.

      (Schroder-Lang et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      A norpA36 background blocks phototaxis in Zzzz\PACαScer\UAS.cSLa-Scer\GAL4Or83b.2.642.T:Hsim\VP22 mutants.

      (Bellmann et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Egra\PACαScer\UAS.cSLa
      Egra\PACαUAS.cSLa
      Zzzz\PACαScer\UAS.cSLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Schroder-Lang
      Saccharyomyces cerevisiae UAS construct a of Schroder-Lang
      Secondary FlyBase IDs
      • FBal0320551
      • FBal0247674
      References (11)