FlyBase Allele Report: Dmel\sona[UAS.cKa]

General Information

Symbol

Dmel\sona^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Kim

FlyBase ID

FBal0322727

Feature type

allele

Associated gene

Dmel\sona

Associated Insertion(s)

Carried in Construct

P{UAS-sona.K}

Also Known As

UAS-sona

Key Links

Transgenic product class

cDNA

(Kim et al., 2016)

wild_type

(Kim et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kim et al., 2016)

wild_type

(Kim et al., 2016)

Mutagen

in vitro construct

(Kim et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-sona.K}

Cytology

Description

UAS regulates expression of sona cDNA.

(Kim et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

sona

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval tracheal section | larval stage, with Scer\GAL4^NP5284

(Kim et al., 2016)

eye, with Scer\GAL4^GMR.PU

(Kim et al., 2016)

ocellar bristle, with Scer\GAL4^wg.PU

(Kim et al., 2016)

wing, with Scer\GAL4^nub.PU

(Kim et al., 2016)

wing, with Scer\GAL4^wg.PU

(Kim et al., 2016)

Detailed Description

Statement

Reference

Expression of sona^Scer\UAS.cKa under the control of any of the following: Scer\GAL4^en.PU, Scer\GAL4^ptc.PU, Scer\GAL4^nub.PU, Scer\GAL4^GMR.PU, Scer\GAL4^wg.PU, Scer\GAL4^30A and Scer\GAL4^sona-NP5284 results in increased lethality at various stages of development and/or loss or malformation of appendages: Expression under the Scer\GAL4^nub.PU driver leads to wing loss, to eye loss under the Scer\GAL4^GMR.PU driver, to small wings and disrupted ocellar bristles under Scer\GAL4^wg.PU and to impaired larval trachea and small and weak adults when expressed under Scer\GAL4^sona-NP5284.

Expression of sona^Scer\UAS.cKa under the control of Scer\GAL4^dpp.PU as well as its transient expression under the control of Scer\GAL4^nub.PU (using UAS-Gal80[ts] to limit the time of expression to late third larval instar/early pupa stage) has no effect on adult wing morphology.

(Kim et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^30A, sona^UAS.cKa has increased mortality during development phenotype, suppressible by Scer\GAL4^30A/arr^GD121

(Han et al., 2020)

Suppressor of

Statement

Reference

sona^UAS.cKa/Scer\GAL4^dpp.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^dpp.PU, wls^GD2418

(Kim et al., 2016)

Other

Statement

Reference

Scer\GAL4^30A, arr^GD121, sona^UAS.cKa has semi-fertile phenotype

(Han et al., 2020)

Scer\GAL4^nub.PU, sona^UAS.cKa, wg^UAS.GFP has visible phenotype

(Kim et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

sona^UAS.cKa/Scer\GAL4^dpp.PU is a suppressor of wing blade phenotype of Scer\GAL4^dpp.PU, wls^GD2418

(Kim et al., 2016)

Other

Statement

Reference

Scer\GAL4^nub.PU, sona^UAS.cKa, wg^UAS.GFP has wing margin bristle | ectopic phenotype

(Kim et al., 2016)

Scer\GAL4^nub.PU, sona^UAS.cKa, wg^UAS.GFP has wing blade phenotype

(Kim et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The notched wing phenotype observed in flies expressing wls^GD2418 under the control of Scer\GAL4^dpp.PU is fully rescued by co-expression of sona^Scer\UAS.cKa.

Co-expression of both wg^{Scer\UAS.T:Avic\GFP} and sona^Scer\UAS.cKa simultaneously under the control of Scer\GAL4^nub.PU (using UAS-Gal80[ts] to limit the time of expression to late third larval instar/early pupa stage) causes high degree of lethality and the surviving adults display wing phenotypes that include multiple ectopic bristles near the anterior wing margin neither of which is observed when wg^{Scer\UAS.T:Avic\GFP} or sona^Scer\UAS.cKa is expressed alone.

(Kim et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

sona^UAS.cKa/Scer\GAL4^nub.PU partially rescues sona⁴⁷

(Kim et al., 2016)

sona^UAS.cKa/Scer\GAL4^ptc.PU partially rescues sona⁴⁷

(Kim et al., 2016)

Comments

Expression of sona^Scer\UAS.cKa under the control of either Scer\GAL4^nub.PU or Scer\GAL4^ptc.PU partially rescues the lethality of sona⁴⁷ homozygous mutants.

(Kim et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sona^Scer\UAS.cKa

sona^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Kim

Secondary FlyBase IDs

References (5)

Report Sections

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