FlyBase Allele Report: Dmel\Dop2R[Δ1]

General Information

Symbol

Dmel\Dop2R^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0322964

Feature type

allele

Associated gene

Dmel\Dop2R

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Scholz-Kornehl and Schwärzel, 2016)

Progenitor genotype

PBac{WH}Dop2R^f06521

(Scholz-Kornehl and Schwärzel, 2016)

PBac{WH}f02905

(Scholz-Kornehl and Schwärzel, 2016)

Caused by aberration

Df(1)Dop2R^Δ1

Cytology

Description

32,386bp deletion that removes part of Dop2R (and all of the neighbouring CG17003 gene).

(Scholz-Kornehl and Schwärzel, 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage

(Scholz-Kornehl and Schwärzel, 2016)

abnormal memory | adult stage (with Df(1)BSC587)

(Scholz-Kornehl and Schwärzel, 2016)

abnormal memory | adult stage (with Dop2R^Δ2)

(Scholz-Kornehl and Schwärzel, 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult Dop2R^Δ1 homozygotes as well as Dop2R^Δ1/Df(1)BSC587 and Dop2R^Δ1/Dop2R^Δ2 transheterozygotes show significantly decreased 3 min short-term memory in an aversive olfactory memory assay compared to controls. The shock and odor avoidance of Dop2R^Δ1 homozygotes is normal. Dop2R^Δ1 homozygotes show decreased memory due to strongly reduced consolidated (amnestic cooling resistant) memory at both 3 hr and 5 min post-training (the labile, amnestic cooling sensitive, memory is unaffected).

(Scholz-Kornehl and Schwärzel, 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Dop2R^Δ1 has abnormal memory | adult stage phenotype, non-enhanceable by Pde4^RNAi.UAS.WIZ/Scer\GAL4^elav.PU

(Scholz-Kornehl and Schwärzel, 2016)

Other

Statement

Reference

Adcy1^RNAi.UAS.cUa, Dop2R^Δ1, Scer\GAL4^elav.PU has abnormal memory | adult stage phenotype

(Scholz-Kornehl and Schwärzel, 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced 3 hr olfactory memory characteristic for Dop2R^Δ1 homozygous adults is decreased further by expression of rut^VDRC.cUa under the control of Scer\GAL4^elav.PU in the mutant background as the amnestic cooling sensitive memory component is reduced in addition to the amnesia-resistant component. Neither the total olfactory memory of the mutants nor any of its components (amnesia resistant or sensitive) is affected by expression of dnc^{dsRNA.Scer\UAS.WIZ}.

(Scholz-Kornehl and Schwärzel, 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dop2R^Δ1 is rescued by Scer\GAL4^elav.PU/Dop2R^UAS.cSa

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^Δ1 is rescued by Scer\GAL4^Mef2.247/Dop2R^UAS.cSa

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^Δ1 is rescued by Scer\GAL4^17d/Dop2R^UAS.cSa

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^Δ1 is rescued by Scer\GAL4^NP1131/Dop2R^UAS.cSa

(Scholz-Kornehl and Schwärzel, 2016)

Not rescued by

Dop2R^Δ1 is not rescued by Scer\GAL4^ple.PF/Dop2R^UAS.cSa

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^Δ1 is not rescued by Dop2R^UAS.cSa/Scer\GAL4^Orco.PU

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^Δ1 is not rescued by Scer\GAL4^GH298/Dop2R^UAS.cSa

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^Δ1 is not rescued by Scer\GAL4^GH146/Dop2R^UAS.cSa

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^Δ1 is not rescued by Dop2R^UAS.cSa/Scer\GAL4^c320

(Scholz-Kornehl and Schwärzel, 2016)

Comments

The defective short-term memory (3 min) displayed by Dop2R^Δ1 homozygotes in an aversive olfactory assay is fully rescued by expression of Dop2R^Scer\UAS.cSa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^Mef2.247 (together with tub-Gal80[ts] to limit the expression to adult stage) in the mutant background.

The decrease in 3 hr olfactory memory due to reduced amnestic cooling resistant memory component also observed in the Dop2R^Δ1 mutants is rescued by expression of Dop2R^Scer\UAS.cSa under the control of any of the following: Scer\GAL4^Mef2.247, Scer\GAL4^17d or Scer\GAL4^NP1131, whereas expression under any of these drivers: Scer\GAL4^ple.PF, Scer\GAL4^Orco.PU, Scer\GAL4^GH298, Scer\GAL4^GH146, Scer\GAL4^c320 has no effect on the mutant phenotype.