FlyBase Allele Report: Dmel\Dop2R[UAS.cSa]

General Information

Symbol

Dmel\Dop2R^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Scholz-Kornehl

FlyBase ID

FBal0322966

Feature type

allele

Associated gene

Dmel\Dop2R

Associated Insertion(s)

Carried in Construct

P{UAS-Dop2R.S}

Key Links

Transgenic product class

cDNA

(Scholz-Kornehl and Schwärzel, 2016)

wild_type

(Scholz-Kornehl and Schwärzel, 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Scholz-Kornehl and Schwärzel, 2016)

wild_type

(Scholz-Kornehl and Schwärzel, 2016)

Mutagen

in vitro construct

(Scholz-Kornehl and Schwärzel, 2016)

Progenitor genotype

Carried in construct

P{UAS-Dop2R.S}

Cytology

Description

UASt regulatory sequences drive expression of a Dop2R cDNA.

(Scholz-Kornehl and Schwärzel, 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dop2R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/Dop2R^UAS.cSa rescues Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Scer\GAL4^Mef2.247/Dop2R^UAS.cSa rescues Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Scer\GAL4^17d/Dop2R^UAS.cSa rescues Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Scer\GAL4^NP1131/Dop2R^UAS.cSa rescues Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Fails to rescue

Scer\GAL4^ple.PF/Dop2R^UAS.cSa fails to rescue Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^UAS.cSa/Scer\GAL4^Orco.PU fails to rescue Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Scer\GAL4^GH298/Dop2R^UAS.cSa fails to rescue Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Scer\GAL4^GH146/Dop2R^UAS.cSa fails to rescue Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Dop2R^UAS.cSa/Scer\GAL4^c320 fails to rescue Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Comments

The defective short-term memory (3 min) displayed by Dop2R^Δ1 homozygotes in an aversive olfactory assay is fully rescued by expression of Dop2R^Scer\UAS.cSa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^Mef2.247 (together with tub-Gal80[ts] to limit the expression to adult stage) in the mutant background.

The decrease in 3 hr olfactory memory due to reduced amnestic cooling resistant memory component also observed in the Dop2R^Δ1 mutants is rescued by expression of Dop2R^Scer\UAS.cSa under the control of any of the following: Scer\GAL4^Mef2.247, Scer\GAL4^17d or Scer\GAL4^NP1131, whereas expression under any of these drivers: Scer\GAL4^ple.PF, Scer\GAL4^Orco.PU, Scer\GAL4^GH298, Scer\GAL4^GH146, Scer\GAL4^c320 has no effect on the mutant phenotype.