FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Lgr3ag8
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General Information
Symbol
Dmel\Lgr3ag8
Species
D. melanogaster
Name
FlyBase ID
FBal0323562
Feature type
allele
Associated gene
Dmel\Lgr3
Associated Insertion(s)
TI{TI}Lgr3ag8
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Garelli et al., 2015)
Progenitor genotype
Associated Insertion(s)
TI{TI}Lgr3ag8
Cytology
Description

A superfolder GFP (sfGFP) coding sequence followed by a flexible linker spacer sequence (GSGSGS) has been inserted into the endogenous Lgr3 locus at the N-terminal end of the Lgr3 open reading frame. The Lgr3 open reading frame contains a bp deletion in the Lgr3 sequence just downstream of the site of the insertion, resulting in a premature termination codon. There is a 350bp deletion in the Lgr3 5'UTR, upstream of the sfGFP insertion, removing the ATG codon and part of the sfGFP sequence.

(Garelli et al., 2015)
Allele components
Component
Use(s)
Tagged with
sfGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:25,667,226..25,667,226 [+]
Comment:

CRISPR/Cas9 knock-in mutation is a 1 bp deletion in the tagged Lgr3 coding sequence, resulting in early translation termination.

(Garelli et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Lgr3ag8/Lgr3ag8 mutants exhibit a significantly shorter time to pupariation in larvae synchronized at the second to third instar molt, as compared to controls.

      (Garelli et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Ilp8Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4arm.PS in a Lgr3ag1/+ background results in delay to pupariation, as compared to controls, but this delay is partially suppressed in a Lgr3ag1/Lgr3ag8 background.

      (Garelli et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Lgr3ag8
      (Garelli et al., 2015)
      ag8
      (Garelli et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)