FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PGRP-LCUAS.LCx.Venus
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General Information
Symbol
Dmel\PGRP-LCUAS.LCx.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0324737
Feature type
allele
Associated gene
Dmel\PGRP-LC
Associated Insertion(s)
Carried in Construct
P{UAS-PGRP-LCx.Venus}
Key Links
Transgenic product class
cDNA
(Harris et al., 2015)
wild_type
(Harris et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Harris et al., 2015)
wild_type
(Harris et al., 2015)
Mutagen
in vitro construct
(Harris et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-PGRP-LCx.Venus}
Cytology
Description

UAS regulatory sequences drive expression of the x isoform of PGRP-LC (open reading frame derived from the IP15793 cDNA clone), tagged at the C-terminal end with Venus.

(Harris et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
PGRP-LC
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of PGRP-LCScer\UAS.LCx.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4VGlut-OK371 leads to a small but significant increase in the spontaneous miniature excitatory postsynaptic potential amplitude, but not of the excitatory postsynaptic potential, in third instar larval neuromuscular junctions, as compared to controls; expression under the control of Scer\GAL4Mhc.PW leads to decreased spontaneous miniature excitatory postsynaptic potential amplitude and decreased excitatory postsynaptic potential amplitude in third instar larval neuromuscular junctions, as compared to controls.

      Expression of PGRP-LCScer\UAS.LCx.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Mhc.PW, but not of Scer\GAL4VGlut-OK371, results in significantly decreased muscle input resistance and decreased negative resting membrane potential in third instar larval neuromuscular junctions, as compared to controls.

      (Harris et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4elav-C155/PGRP-LCUAS.LCx.Venus partially rescues PGRP-LCΔE

      (Harris et al., 2015)

      Scer\GAL4VGlut1-OK371/PGRP-LCUAS.LCx.Venus partially rescues PGRP-LCΔE

      (Harris et al., 2015)
      Fails to rescue

      PGRP-LCUAS.LCx.Venus/Scer\GAL4Mhc.PW fails to rescue PGRP-LCΔE

      (Harris et al., 2015)
      Comments

      Expression of PGRP-LCScer\UAS.LCx.T:Avic\GFP-YFP.Venus under the control of either Scer\GAL4elav-C155 or Scer\GAL4VGlut-OK371, but not of Scer\GAL4Mhc.PW, rescues the decreased spontaneous miniature excitatory postsynaptic potential observed in neuromuscular junctions of PGRP-LCΔE homozygous mutant third instar larvae; expression of PGRP-LCScer\UAS.LCx.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4elav-C155, but not of Scer\GAL4VGlut-OK371 or Scer\GAL4Mhc.PW, also rescues the decreased excitatory postsynaptic potential observed in neuromuscular junctions of PGRP-LCΔE homozygous mutant third instar larvae.

      (Harris et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PGRP-LCScer\UAS.LCx.T:Avic\GFP-YFP.Venus
      PGRP-LCUAS.LCx.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (1)