FlyBase Allele Report: Dmel\PGRP-LC[ΔE]

General Information

Symbol

Dmel\PGRP-LC^ΔE

Species

D. melanogaster

Name

FlyBase ID

FBal0212184

Feature type

allele

Associated gene

Dmel\PGRP-LC

Associated Insertion(s)

P{XP-U}PGRP-LC^ΔE

Carried in Construct

Also Known As

PGRP-LC^E12, PGRP-LC^ΔE12, PGRP-LC^DE12, LC^ΔE

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Gottar et al., 2002)

Mutagen

P-element activity

(Gottar et al., 2002)

recombination

(Gottar et al., 2002)

Progenitor genotype

P{XP}4396

(Gottar et al., 2002)

P{XP}PGRP-LC⁰⁶⁹³

(Gottar et al., 2002)

Associated Insertion(s)

P{XP-U}PGRP-LC^ΔE

Cytology

Description

Recombination between P{XP}PGRP-LC⁰⁶⁹³ and P{XP}4396, resulting in an 8.7kb deletion which removes the entire PGRP-LC coding region.

(Gottar et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{XP-U}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult anterior midgut

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number

(Jugder et al., 2021)

adult proventriculus | conditional

(Charroux et al., 2018)

embryonic/larval neuromuscular junction | third instar larval stage

(Harris et al., 2015)

embryonic/larval neuromuscular junction | third instar larval stage (with PGRP-LC²)

(Harris et al., 2015)

lipid droplet | adult stage

(Jugder et al., 2021)

Detailed Description

Statement

Reference

PGRP-LC^ΔE mutants exhibit fewer enteroendocrine cells and more lipid droplets in the anterior midgut,

(Jugder et al., 2021)

PGRP-LC^ΔE adults infected with Erwinia carotovora carotovora by oral feeding exhibit an absence of immune response (immune deficiency pathway activation) in proventriculus compared to controls.

(Charroux et al., 2018)

PGRP-LE¹¹², PGRP-LC^ΔE double homozygotes show a significant increase in viral load in the head upon infection with ZIKV, as compared to infected double heterozygotes.

(Liu et al., 2018)

PGRP-LC^ΔE mutant flies display increased sensitivity to systemic Escherichia coli infection.

(Paik et al., 2017)

PGRP-LC^ΔE homozygous adults exhibit an impaired capacity to eliminate bacteria and a significantly increased mortality after abdominal infection with Erwinia carotovora carotovora, as compared to controls.

(Neyen et al., 2016)

The neuromuscular junctions of PGRP-LC^ΔE homozygous mutant third instar larvae present small but significant decreases in spontaneous miniature excitatory postsynaptic potential and in excitatory postsynaptic potential, but not in quantal content, as compared to controls.

The neuromuscular junctions of PGRP-LC^ΔE/PGRP-LC² transheterozygous mutant third instar larvae present small but significant decrease in spontaneous miniature excitatory postsynaptic potential and increase in quantal content, but no significant effect in the excitatory postsynaptic potential, as compared to controls.

The neuromuscular junctions of PGRP-LC^ΔE homozygous mutant third instar larvae shows similar overall structure to controls, with similar number of synaptic boutons, similar number and length of active zones, similar average vesicle number, similar vesicle diameter, similar average vesicle distance from the T-bar centroid.

(Harris et al., 2015)

Mutant flies show reduced compared to wild type after injection with Ecc15 (Gram-negative bacterium).

(Gendrin et al., 2013)

PGRP-LC^ΔE mutants show no effect on mortality within 24 hours of mild wounding in 7-8 days old adults. The mortality rate is increased to ~50% in response to more severe wounding.

(Nam et al., 2012)

Mutant flies infected with Erwinia carotovora carotovora by septic injury rapidly succumb to the infection.

(Neyen et al., 2012)

PGRP-LC^ΔE mutant flies do not show a systemic response to genital infection with E.carotovora.

(Gendrin et al., 2009)

PGRP-LC^ΔE mutants are susceptible to infection by Gram-negative bacteria.

(Maillet et al., 2008)

Hemolymph clots from PGRP-LC^ΔE third instar larvae show normal melanization.

(Bidla et al., 2007)

Mutant flies show similar survival rates as control flies following natural infection (feeding with contaminated food) with either "Ecc-15" (P.carotovorum.carotovorum), S.cerevisiae or M.luteus.

(Ha et al., 2005)

PGRP-LC^E12 flies show a normal survival rate after natural infection with "Ecc15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

Mutant flies show reduced survival compared to wild-type controls after infection with E. coli.

(Bischoff et al., 2004)

Succumbs rapidly to E. faecalis (Gram-positive bacteria) infection, but not B. Bassiana (natural fungus) infection.

(Gobert et al., 2003)

PGRP-LC^ΔE flies show a marked susceptibility to infection by Agrobacterium tumefaciens.

(Gottar et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

PGRP-LC^ΔE has abnormal immune response | adult stage phenotype, enhanceable by PGRP-LE¹¹²

(Paik et al., 2017)

PGRP-LC^ΔE has abnormal immune response | adult stage phenotype, enhanceable by CG8046¹

(Paik et al., 2017)

Suppressor of

Statement

Reference

PGRP-LC^ΔE is a suppressor | partially of abnormal immune response | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

PGRP-LC^ΔE/PGRP-LC^ΔE is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of GluRIIA^SP16

(Harris et al., 2015)

PGRP-LC^ΔE is a suppressor of visible | adult stage phenotype of PGRP-LF^RNAi.1.UAS, Scer\GAL4^ptc-559.1

(Maillet et al., 2008)

PGRP-LC^ΔE is a suppressor of abnormal immune response phenotype of PGRP-SC1a^RNAi.UAS, Scer\GAL4^da.G32

(Bischoff et al., 2006)

Other

Statement

Reference

PGRP-LC^ΔE, PGRP-LE¹¹² has abnormal immune response | recessive | adult stage phenotype

(Liu et al., 2018)

PGRP-LC^ΔE, PGRP-LE¹¹² has abnormal immune response phenotype

(Aggarwal et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

PGRP-LC^ΔE is a suppressor | partially of adult anterior midgut | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

PGRP-LC^ΔE is a suppressor | partially of adult fat body | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

PGRP-LC^ΔE/PGRP-LC^ΔE is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of GluRIIA^SP16

(Harris et al., 2015)

PGRP-LC^ΔE is a suppressor of wing phenotype of PGRP-LF^RNAi.1.UAS, Scer\GAL4^ptc-559.1

(Maillet et al., 2008)

NOT Suppressor of

Statement

Reference

PGRP-LC^ΔE is a non-suppressor of adult posterior midgut | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

PGRP-LC^ΔE is a non-suppressor of adult midgut region R3 | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of PGRP-LC^ΔE and PGRP-LE¹¹² leads to an absence of immune deficiency pathway activation in proventriculus of adults infected with Erwinia carotovora carotovora by oral feeding different than controls.

(Charroux et al., 2018)

The reduced survival rate upon systemic infection with Escherichia coli observed in PGRP-LC^ΔE mutants is substantially decreased further in CG8046¹;PGRP-LC^ΔE as well as PGRP-LE¹¹²;PGRP-LC^ΔE double mutants.

(Paik et al., 2017)

PGRP-LC^ΔE, PGRP-LE¹¹² double mutants do not exhibit any difference in survival rate of flies either untreated or treated with Leishmania amastigotes, as compared to wild type.

(Okuda et al., 2016)

PGRP-LC^ΔE homozygosity reverses the decrease in spontaneous miniature excitatory postsynaptic potential and increase in quantal content observed in the neuromuscular junction of GluRIIA^SP16 homozygous mutant third instar larvae.

(Harris et al., 2015)

PGRP-LE¹¹²;PGRP-LC^ΔE double mutant flies are rapidly killed by infection with the gram-negative pathogen Erwinia carotovora carotovora.

(Aggarwal et al., 2008)

A PGRP-LC^ΔE background suppresses the wing notches seen when PGRP-LF^{dsRNA.1.Scer\UAS} is expressed under the control of Scer\GAL4^ptc-559.1.

(Maillet et al., 2008)

The increase in lethality seen in larvae expressing PGRP-SC1a^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^da.G32) is completely suppressed in a PGRP-LC^ΔE background.

(Bischoff et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PGRP-LC^ΔE is rescued by PGRP-LC^+tNa

(Jugder et al., 2021)

PGRP-LC^ΔE is rescued by PGRP-LC^EGFP

(Neyen et al., 2016)

PGRP-LC^ΔE is rescued by Scer\GAL4^VGlut1-OK371/PGRP-LC^{ΔC.UAS.LCx.Venus}

(Harris et al., 2015)

PGRP-LC^ΔE is rescued by PGRP-LC^EGFP

(Neyen et al., 2012)

PGRP-LC^ΔE is rescued by PGRP-LC^LCx

(Neyen et al., 2012)

PGRP-LC^ΔE is rescued by PGRP-LC^+tNa

(Neyen et al., 2012)

PGRP-LC^ΔE is rescued by Scer\GAL4^yolk/PGRP-LC^A.TM+IC.UAS

(Maillet et al., 2008)

Partially rescued by

PGRP-LC^ΔE is partially rescued by PGRP-LC^Δex5.EGFP

(Neyen et al., 2016)

PGRP-LC^ΔE is partially rescued by Scer\GAL4^elav-C155/PGRP-LC^{UAS.LCx.Venus}

(Harris et al., 2015)

PGRP-LC^ΔE is partially rescued by Scer\GAL4^VGlut1-OK371/PGRP-LC^{UAS.LCx.Venus}

(Harris et al., 2015)

Not rescued by

PGRP-LC^ΔE is not rescued by PGRP-LC^LCa

(Jugder et al., 2021)

PGRP-LC^ΔE is not rescued by PGRP-LC^LCy

(Jugder et al., 2021)

PGRP-LC^ΔE is not rescued by PGRP-LC^{UAS.LCx.Venus}/Scer\GAL4^Mhc.PW

(Harris et al., 2015)

PGRP-LC^ΔE is not rescued by PGRP-LC^LCa

(Neyen et al., 2012)

PGRP-LC^ΔE is not rescued by PGRP-LC^LCy

(Neyen et al., 2012)

PGRP-LC^ΔE is not rescued by PGRP-LC^LCa/PGRP-LC^LCy

(Neyen et al., 2012)

PGRP-LC^ΔE is not rescued by Scer\GAL4^yolk/PGRP-LC^A.EC.UAS

(Maillet et al., 2008)

PGRP-LC^ΔE is not rescued by Scer\GAL4^yolk/PGRP-LC^A.TM+EC.UAS

(Maillet et al., 2008)

Comments

PGRP-LC^ΔE homozygous adults exhibit an impaired capacity to eliminate bacteria and a significantly increased mortality upon abdominal infection with Erwinia carotovora carotovora, which are fully rescued by PGRP-LC^{T:Avic\GFP-EGFP} or fully and partially rescued, respectively, by PGRP-LC^{Δex5.T:Avic\GFP-EGFP}; The progeny of PGRP-LC^ΔE homozygous female adults harboring either PGRP-LC^{T:Avic\GFP-EGFP} or PGRP-LC^{Δex5.T:Avic\GFP-EGFP} do not exhibit significant lethality as compared to the progeny of control females.

(Neyen et al., 2016)

Expression of PGRP-LC^{Scer\UAS.LCx.T:Avic\GFP-YFP.Venus} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^VGlut-OK371, but not of Scer\GAL4^Mhc.PW, rescues the decreased spontaneous miniature excitatory postsynaptic potential observed in neuromuscular junctions of PGRP-LC^ΔE homozygous mutant third instar larvae; expression of PGRP-LC^{Scer\UAS.LCx.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^elav-C155, but not of Scer\GAL4^VGlut-OK371 or Scer\GAL4^Mhc.PW, also rescues the decreased excitatory postsynaptic potential observed in neuromuscular junctions of PGRP-LC^ΔE homozygous mutant third instar larvae.

Expression of PGRP-LC^{ΔC.Scer\UAS.LCx.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^VGlut-OK371 rescues the decreased spontaneous miniature excitatory postsynaptic potential and decreased excitatory postsynaptic potential observed in neuromuscular junctions of PGRP-LC^ΔE homozygous mutant third instar larvae.

(Harris et al., 2015)

PGRP-LC^+tNa (carried either in M{PGRP-LC.PGRP-LF} or in M{PGRP-LC}) rescues the susceptibility of PGRP-LC^ΔE flies to infection with Erwinia carotovora carotovora by septic injury.

PGRP-LC^{T:Avic\GFP-EGFP} (carried in M{GFP-PGRP-LC.PGRP-LF}) rescues the susceptibility of PGRP-LC^ΔE flies to infection with Erwinia carotovora carotovora by septic injury.

PGRP-LC^LCa and PGRP-LC^LCy (either alone or in combination) fail to rescue the susceptibility of PGRP-LC^ΔE flies to infection with Erwinia carotovora carotovora by septic injury.

(Neyen et al., 2012)

Expression of PGRP-LC^{A.TM+IC.Scer\UAS} under the control of Scer\GAL4^yolk rescues the susceptibility of PGRP-LC^ΔE mutants to infection by Gram-negative bacteria.

Expression of PGRP-LC^{A.EC.Scer\UAS} under the control of Scer\GAL4^yolk does not rescue the susceptibility of PGRP-LC^ΔE mutants to infection by Gram-negative bacteria.

Expression of PGRP-LC^{A.TM+EC.Scer\UAS} under the control of Scer\GAL4^yolk does not rescue the susceptibility of PGRP-LC^ΔE mutants to infection by Gram-negative bacteria.

(Maillet et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

55713

w^*; P{XP-U}PGRP-LC^ΔE

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

LC^Δ

(Kenmoku et al., 2017)

LC^ΔE

(Nagai et al., 2021, Liu et al., 2018)

PGRP-LC&Dgr

(Vincow et al., 2024)

PGRP-LC^DE12

(Bosco-Drayon et al., 2012, Akhouayri et al., 2011)

PGRP-LC^DeltaE

(Ferrandon, 2007.11.30)

PGRP-LC^del

(Harris et al., 2015)

PGRP-LC^ΔE12

(Zhang et al., 2024, Kurz et al., 2017, Costechareyre et al., 2016, Bosco-Drayon et al., 2012)

PGRP-LC^ΔE

(Harsh et al., 2019, Nandy et al., 2018, Paik et al., 2017, Aggarwal et al., 2008, Davis et al., 2008, Bischoff et al., 2006, Kim et al., 2006, Bischoff et al., 2004)

PGRP-LCE12

(Chen et al., 2022)

PGRP^del

(Harris et al., 2015)

pgrp lc^ΔE

(Okuda et al., 2016)

ΔLC^E12

(Neyen et al., 2016)

Name Synonyms

Secondary FlyBase IDs

FBal0135886
FBal0156116

References (55)

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