FB2025_05 , released December 11, 2025
Allele: Dmel\Nprl21
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General Information
Symbol
Dmel\Nprl21
Species
D. melanogaster
Name
FlyBase ID
FBal0325026
Feature type
allele
Associated gene
Dmel\Nprl2
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Wei et al., 2016)
Mutagen
CRISPR/Cas9
(Wei et al., 2016)
Progenitor genotype
Cytology
Description

Deletion of most of the Nprl2 coding region, deletion contains 30 amino acids from the N-terminus and four frame-shifted amino acids from the C-terminus.

(Wei et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Nprl21 homozygotes are partially lethal and majority die as pharate adults. Surviving adult show reduced climbing ability.

      The size of cells in the Nprl21 somatic follicle cell clones is significantly increased compared to adjacent heterozygous cells and the egg chambers containing Nprl21 mutant germline clones are also increased in size.

      Nprl21 mutant adults display reduced survival rates under nutrient -liming conditions (complete starvation as well as amino acid starvation) and fail to activate autophagy (assessed by LysoTracker staining in larval fat body) upon starvation.

      (Wei et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Nprl21 has partially lethal - majority die phenotype, non-enhanceable by Nprl31

      (Wei et al., 2016)
      Suppressed by
      Statement
      Reference

      Nprl21 has partially lethal - majority die phenotype, suppressible | partially by Tor[+]/mTorA948V

      (Wei et al., 2016)
      NOT Enhancer of
      Statement
      Reference

      Nprl21 is a non-enhancer of partially lethal - majority die phenotype of Nprl31

      (Wei et al., 2016)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The partial lethality of either Nprl21 or Nprl31 single mutants is not increased further in the Nprl21;Nprl31 double mutants but can be significantly suppressed by combination with a single copy of TorA948V.

      (Wei et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Nprl21 is rescued by Nprl2UASp.Tag:FLAG,Tag:HA/Scer\GAL4da.G32

      (Wei et al., 2016)
      Comments

      The partial lethality, reduced climbing ability and survival rates under nutrient-limiting conditions of the surviving adults as well as the failure to activate autophagy upon starvation (assessed by Lysotracker staining in larval fat body) characteristic for Nprl21 homozygotes is rescued by expression of Nprl2Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4da.G32 in the mutant background.

      (Wei et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Nprl21
      (Zhang et al., 2023)
      nprl21
      (Jouandin et al., 2022, Zhou et al., 2021, Wei et al., 2019, Xi et al., 2019, Wei et al., 2016)
      nprl1
      (Gu et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)