FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Poc1W87X
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General Information
Symbol
Dmel\Poc1W87X
Species
D. melanogaster
Name
FlyBase ID
FBal0325419
Feature type
allele
Associated gene
Dmel\Poc1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Khire et al., 2016)
Progenitor genotype
Cytology
Description

Amino acid replacement: W87term.

(Khire et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:13,389,613..13,389,613 [-]
Nucleotide change:

G13389613A

Amino acid change:

W87term | Poc1-PA; W87term | Poc1-PB

Reported amino acid change:

W87term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Khire et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous Poc1W87X adults display morphological sperm defects (immotile spermatozoa with abnormal axoneme structure, shorter giant centriole compared to controls) but their locomotor behavior appears unaffected (suggesting sensory cilia function is not perturbed).

      (Khire et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of ana2Ubi.C.GFP in combination with Sas-6Ubi.P.GFP leads to formation of procentriolar ectopic particles while their expression in Poc1W87X homozygous background leads to structural defects in procentriolar ectopic particles such as fragmented tubule-like structures and electron-dense material in mature primary spermatocytes.

      (Jo et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Poc1W87X is rescued by Poc1ABGFP

      (Khire et al., 2016)

      Poc1W87X is rescued by Poc1UAS.B.GFP/Poc1UAS.A.GFP/Scer\GAL4bam.PU

      (Khire et al., 2016)
      Partially rescued by

      Poc1W87X is partially rescued by Poc1UAS.A.GFP/Scer\GAL4bam.PU

      (Khire et al., 2016)
      Not rescued by

      Poc1W87X is not rescued by Poc1UAS.B.GFP/Scer\GAL4bam.PU

      (Khire et al., 2016)
      Comments

      Expression of Poc1UAS.A.GFP alone under the control of Scer\GAL4bam.PU in the Poc1W87X mutant background rescues the sperm motility defect characteristic for Poc1W87X males but not the reduced size of the giant centriole in the sperm cells. Expression of Poc1UAS.B.GFP alone cannot rescue either of the phenotypes but simultaneous expression of both Poc1UAS.A.GFP and Poc1UAS.B.GFP using the same driver rescues both phenotypes and the progeny of these males show nearly normal embryonic development rate compared (unlike the embryos fathered by Poc1W87X mutants expressing only Poc1UAS.A.GFP which are severely delayed).

      (Khire et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      3-3416
      (Khire et al., 2016)
      Poc1W87X
      (Jo et al., 2019)
      poc1W87X
      (Khire et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)