UASp regulatory sequences drive expression of the 5'UTR and coding sequence of the Tm1 I isoform. The protein is tagged at the N-terminal end with Emerald. Tm1 3'UTR sequences, derived from an ovarian cDNA, are also present.
Scer\GAL4VP16.osk/Tm1UASp.I.Emerald rescues Tm1eg9/Tm1eg1
Expression of Tm1Scer\UAS.P\T.I.T:Avic\GFP-EMD under the control of Scer\GAL4osk.T:Hsim\VP16 rescues the grandchildless phenotype of Tm1eg9/Tm1eg1 and restores the posterior pole localization of osk mRNA in the mutant oocytes.