FlyBase Allele Report: Dmel\TpnC4[δ25]

General Information

Symbol

Dmel\TpnC4^δ25

Species

D. melanogaster

Name

FlyBase ID

FBal0325810

Feature type

allele

Associated gene

Dmel\TpnC4

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chechenova et al., 2017)

Mutagen

CRISPR/Cas9

(Chechenova et al., 2017)

Progenitor genotype

Cytology

Description

2bp deletion plus a 9bp insertion in exon 3 of TpnC4; the insertion carries a stop codon, resulting in translation termination after the sixth amino acid of the polypeptide.

(Chechenova et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2R:5,506,778..5,506,779 [-]

Inserted_sequence:

AATTGAATA

Comment:

The deleiton of two bases (GA) and the insertion of nine bases (AATTGAATA - which includes an in-frame stop codon) at this site leads to early translation termination.

(Chechenova et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

TpnC4^δ25/TpnC4^δ25 is an enhancer of abnormal jump response phenotype of TpnC41C^δ2

(Chechenova et al., 2017)

Other

Statement

Reference

TpnC41C^δ2, TpnC4^δ25 has partially lethal - majority die phenotype

(Chechenova et al., 2017)

TpnC41C^δ2, TpnC4^δ25 has flightless phenotype

(Chechenova et al., 2017)

Phenotype Manifest In

Other

Statement

Reference

TpnC41C^δ2, TpnC4^δ25 has myofibril | pharate adult stage phenotype

(Chechenova et al., 2017)

TpnC41C^δ2, TpnC4^δ25 has mesothoracic tergotrochanter muscle cell | pharate adult stage phenotype

(Chechenova et al., 2017)

TpnC41C^δ2, TpnC4^δ25 has indirect flight muscle cell | pharate adult stage phenotype

(Chechenova et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

TpnC41C^δ2,TpnC4^δ25 double homozygous mutants are able to reach late pupal stages but only a few adults successful eclose. These adults have very low mobility, cannot jump and are flightless. The myofibrils of the mesothoracic extracoxal depressor muscle 66 (tergal depressor of the trochanter muscle) are severely disorganized (reduced and patchy Z-line) and while the myofibrils in the indirect flight muscle do retain their overall sarcomere architecture they do not have consistent diameter along their length and the Z-lines are often indistinct in the double mutant pharate adults.

(Chechenova et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

This allele was made in a TpnC41C^δ2 background.

(Chechenova et al., 2017)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

TpnC4^δ#25

(Chechenova et al., 2017)

TpnC4^δ25

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close