FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\lokKD
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General Information
Symbol
Dmel\lokKD
Species
D. melanogaster
Name
FlyBase ID
FBal0325825
Feature type
allele
Associated gene
Dmel\lok
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Ma et al., 2016)
Mutagen
CRISPR/Cas9
(Ma et al., 2016)
Progenitor genotype
Cytology
Description

Amino acid replacement: D286A.

(Ma et al., 2016)

The D286A amino acid replacement (responsible for CHK2 kinase activity) results in a kinase-dead lok.

(Ma et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:20,063,393..20,063,393 [+]
Nucleotide change:

A20063393C

Reported nucleotide change:

A?C

Amino acid change:

D303A | lok-PA; D286A | lok-PB; D286A | lok-PC

Reported amino acid change:

D286A

Comment:

Residue 286 relative to lok-PB was mutated from GCC to GAC (A286D).

(Ma et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Nestor-Guillermo progeria syndrome
      modeled by bafgfpA13T
      (Duan et al., 2023)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      lokKD homo- and heterozygous as well as lokKD/lokp6 females show a much milder loss of germline stem cells upon X-ray irradiation than wild-type controls.

      (Ma et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      lok[+]/lokKD is an enhancer of increased cell number | adult stage | female | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      Suppressor of
      Statement
      Reference

      lokKD/lokKD is a suppressor of increased cell death | oogenesis phenotype of bafgfpA13T

      (Duan et al., 2023)

      lokKD/lokKD is a suppressor of decreased fecundity phenotype of bafgfpA13T

      (Duan et al., 2023)

      lokKD/lokKD is a suppressor | partially of increased mortality during development phenotype of bafgfpA13T

      (Duan et al., 2023)

      lokKD/lokKD is a suppressor of increased cell death | larval stage phenotype of bafgfpA13T

      (Duan et al., 2023)

      lok[+]/lokKD is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokKD/lokKD is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokp6/lokKD is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokKD/lokKD is a suppressor of increased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokp6/lokKD is a suppressor of increased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      lok[+]/lokKD is an enhancer of cystoblast | adult stage | increased number | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      Suppressor of
      Statement
      Reference

      lokKD/lokKD is a suppressor of germarium phenotype of bafgfpA13T

      (Duan et al., 2023)

      lokKD/lokKD is a suppressor of wing disc | larval stage phenotype of bafgfpA13T

      (Duan et al., 2023)

      lok[+]/lokKD is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokKD/lokKD is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokp6/lokKD is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokKD/lokKD is a suppressor of cystoblast | increased number | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)

      lokp6/lokKD is a suppressor of cystoblast | increased number | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreIhs.PR (to introduce DNA damage) is rescued by combination with lokp6 or lokKD in hetero-, homo- or transheterozygosity as these double mutant flies contain significantly higher number of GSCs both at 3 days and 1 week post heat-shock compared to Crei\I-CreIhs.PR single mutants.

      lokKD/+;Crei\I-CreIhs.PR, lokKD/lokKD;Crei\I-CreIhs.PR or lokKD/lokp6;Crei\I-CreIhs.PR mutants have more GSCs than wild-type controls even without the induction of DNA damage by heat-shock.

      Crei\I-CreIhs.PR germaria in which DNA damage has been evoked by heat-shock accumulate cystoblast-like single cells and this accumulation is accelerated by lokp6 or lokKD heterozygosity (significantly increased number of CB-like cells observed already at 3 days post heat-shock). By contrast, both lokp6 or lokKD homo- or transheterozygosity completely abolishes the CB-like cell accumulation phenotype.

      (Ma et al., 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      chk2KD
      (Duan et al., 2023)
      lokKD
      (Easwaran et al., 2022, Ma et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)