FlyBase Allele Report: Crei\I-CreI[hs.PR]

General Information

Symbol

Crei\I-CreI^hs.PR

Species

C. reinhardtii

Name

heat shock construct of Rong

FlyBase ID

FBal0137561

Feature type

allele

Associated gene

Crei\I-CreI

Associated Insertion(s)

Carried in Construct

P{hs-I-CreI.R}

Also Known As

hs-I-CreI, 70I-CreI

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rong et al., 2002)

Progenitor genotype

Carried in construct

P{hs-I-CreI.R}

Cytology

Description

An Hsp70 promoter drives expression of the I-CreI endonuclease.

(Rong et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

I-CreI

meganuclease

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cystoblast | adult stage | increased number | temperature conditional

(Ma et al., 2016)

female germline stem cell | adult stage | temperature conditional

(Ma et al., 2016)

larval brain & neuroblast | conditional ts

(Maggert and Golic, 2005)

primordial germ cell | decreased number | embryonic stage

(Tarayrah-Ibraheim et al., 2021)

Detailed Description

Statement

Reference

Heat-shock induced expression of Crei\I-CreI^hs.PR (to introduce DNA damage) in adult female flies leads to cell cycle arrest in the germline stem cells (GSCs) directly after DNA damage and then the cells resume rapid proliferation. Following the heat-shock, the mutant germaria display a reduced number of GSCs relative to no heat-shock controls and this number stays low even a week after the damage induction. No apoptotic GSCs (visualized by *Casp3 labelling) are detected in the challenged mutant germaria and the size of the nucleolus in the GSCs (indicative of rRNA production) is comparable to controls.

Crei\I-CreI^hs.PR germaria in which DNA damage has been evoked by heat-shock accumulate cystoblast-like single cells compared to undamaged controls.

(Ma et al., 2016)

Expression of Crei\I-CreI^hs.PR using heat shock results in a dramatic drop in the mitotic index of larval brain neuroblasts. Expression of Crei\I-CreI^hs.PR using heat shock results in recombination between the X and Y chromosomes in the heterochromatic regions where the rDNA is located; approximately 20% of the offspring of males expressing Crei\I-CreI^hs.PR show exceptional inheritance of X- and Y-linked markers, consistent with exchange at rDNA loci.

(Maggert and Golic, 2005)

Heat shock induction of Crei\I-CreI^hs.PR produces a high frequency of abnormal anaphases, in which chromosome fagments remain at the metaphase plate. Besides an overall reduction in the mitotic index, the frequency of anaphase cells drops significantly compared to control cells.

(Royou et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Crei\I-CreI^hs.PR has decreased cell number | adult stage | female | temperature conditional phenotype, enhanceable by Scer\GAL4^nanos.PU/mei-41^HMS02331

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | adult stage | female | temperature conditional phenotype, enhanceable by Scer\GAL4^nanos.PU/tefu^HMS02790

(Ma et al., 2016)

Crei\I-CreI^hs.PR has increased cell number | adult stage | female | temperature conditional phenotype, enhanceable by lok[+]/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | adult stage | female | temperature conditional phenotype, enhanceable by p53^5A-1-4/p53^5A-1-4

(Ma et al., 2016)

Crei\I-CreI^hs.PR has increased cell number | adult stage | female | temperature conditional phenotype, enhanceable by bam[+]/bam^Δ86

(Ma et al., 2016)

Crei\I-CreI^hs.PR has increased cell number | adult stage | female | temperature conditional phenotype, enhanceable by lok[+]/lok^p6

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | adult stage | female | temperature conditional phenotype, enhanceable by mei-41[+]/mei-41^D3

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | adult stage | female | temperature conditional phenotype, enhanceable by mei-41^D3/mei-41^D3

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | adult stage | female | temperature conditional phenotype, enhanceable by mei-41^GL00284/Scer\GAL4^nanos.PU

(Ma et al., 2016)

NOT Enhanced by

Statement

Reference

Crei\I-CreI^hs.PR has abnormal mitotic cell cycle phenotype, non-enhanceable by BubR1^unspecified

(Royou et al., 2005)

Suppressed by

Statement

Reference

Crei\I-CreI^hs.PR has decreased cell number | adult stage | temperature conditional phenotype, suppressible by lok[+]/lok^p6

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^p6

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok^GL00020/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok[+]/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok^KD/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by p53^5A-1-4/p53[+]

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by tkv^Q253D.UASp/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by tkv^Q253D.UASp/sax^Q263D.UASp/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by tkv^Q253D.UASp/Scer\GAL4^nanos.PU/shg^{UASp.Tag:FLAG}

(Ma et al., 2016)

Crei\I-CreI^hs.PR has increased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^p6

(Ma et al., 2016)

Crei\I-CreI^hs.PR has increased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok^KD/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has increased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^KD

(Ma et al., 2016)

NOT suppressed by

Statement

Reference

Crei\I-CreI^hs.PR has decreased cell number | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^nanos.PU/BacA\p35^{UASp.Tag:FLAG}

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^nanos.PU/grp^HMS01573

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, non-suppressible by grp^HMC05162/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^nanos.PU/shg^{UASp.Tag:FLAG}

(Ma et al., 2016)

Enhancer of

Statement

Reference

Crei\I-CreI^hs.PR is an enhancer of abnormal mitotic cell cycle phenotype of BubR1^unspecified

(Royou et al., 2005)

Other

Statement

Reference

Crei\I-CreI^hs.PR, mei-41^D3 has decreased cell number | female | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, Scer\GAL4^nanos.PU, tefu^HMS02790 has decreased cell number | female | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, lok[+]/lok^KD has increased cell number | female | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, lok^KD has increased cell number | female | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, lok^p6/lok^KD has increased cell number | female | adult stage phenotype

(Ma et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by Scer\GAL4^nanos.PU/mei-41^HMS02331

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by Scer\GAL4^nanos.PU/tefu^HMS02790

(Ma et al., 2016)

Crei\I-CreI^hs.PR has cystoblast | adult stage | increased number | temperature conditional phenotype, enhanceable by lok[+]/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by p53^5A-1-4/p53^5A-1-4

(Ma et al., 2016)

Crei\I-CreI^hs.PR has cystoblast | adult stage | increased number | temperature conditional phenotype, enhanceable by bam[+]/bam^Δ86

(Ma et al., 2016)

Crei\I-CreI^hs.PR has cystoblast | adult stage | increased number | temperature conditional phenotype, enhanceable by lok[+]/lok^p6

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by mei-41[+]/mei-41^D3

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by mei-41^D3/mei-41^D3

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by mei-41^GL00284/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Suppressed by

Statement

Reference

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok^GL00020/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok[+]/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok^KD/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by p53^5A-1-4/p53[+]

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by tkv^Q253D.UASp/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by tkv^Q253D.UASp/sax^Q263D.UASp/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by tkv^Q253D.UASp/Scer\GAL4^nanos.PU/shg^{UASp.Tag:FLAG}

(Ma et al., 2016)

Crei\I-CreI^hs.PR has cystoblast | increased number | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^p6

(Ma et al., 2016)

Crei\I-CreI^hs.PR has cystoblast | increased number | adult stage | temperature conditional phenotype, suppressible by lok^KD/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has cystoblast | increased number | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^KD

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok[+]/lok^p6

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok^p6/lok^p6

(Ma et al., 2016)

NOT suppressed by

Statement

Reference

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^nanos.PU/BacA\p35^{UASp.Tag:FLAG}

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^nanos.PU/grp^HMS01573

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by grp^HMC05162/Scer\GAL4^nanos.PU

(Ma et al., 2016)

Crei\I-CreI^hs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^nanos.PU/shg^{UASp.Tag:FLAG}

(Ma et al., 2016)

Other

Statement

Reference

Crei\I-CreI^hs.PR, mei-41^D3 has female germline stem cell | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, Scer\GAL4^nanos.PU, tefu^HMS02790 has female germline stem cell | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, lok[+]/lok^KD has female germline stem cell | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, lok^KD has female germline stem cell | adult stage phenotype

(Ma et al., 2016)

Crei\I-CreI^hs.PR, lok^p6/lok^KD has female germline stem cell | adult stage phenotype

(Ma et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The loss of germline stem cells (GSCs) in female germaria observed upon heat-shock-induced expression of Crei\I-CreI^hs.PR (to introduce DNA damage) cannot be rescued by Scer\GAL4^nos.PU-driven expression of BacA\p35^{UASp.Tag:FLAG} in the mutant background.

The damage-evoked loss of GSCs upon expression of Crei\I-CreI^hs.PR (induced by heat-shock to introduce DNA breaks) is rescued by combination with lok^p6 or lok^KD in hetero-, homo- or transheterozygosity as these double mutant flies contain significantly higher number of GSCs both at 3 days and 1 week post heat-shock compared to Crei\I-CreI^hs.PR single mutants. Similarly, Scer\GAL4^nos.PU-driven expression of lok^GL00020 or tkv^{Q253D.Scer\UAS.P\T} or simultaneous expression of both tkv^{Q253D.Scer\UAS.P\T} and either sax^Q263D.UASp or shg^{UASp.Tag:FLAG} also significantly suppresses the damage-evoked GSC loss in Crei\I-CreI^hs.PR flies. In contrast, this loss is further exacerbated by mei-41^D3 in homo- or heterozygosity or by expression of mei-41^GL00284, mei-41^HMS02331 or tefu^HMS02790 under the Scer\GAL4^nos.PU driver, expression of either grp^HMS01573 or grp^HMC05162 or shg^{UASp.Tag:FLAG} has no effect on the GSCs number in Crei\I-CreI^hs.PR mutant background either in the presence or absence of DNA damage. The loss of GSCs in damaged Crei\I-CreI^hs.PR germaria is slightly alleviated by combination with a single copy of p53^5A-1-4 but is actually made worse by p53^5A-1-4 homozygosity.

The number of GSCs remaining after DNA-damage has been induced is slightly higher both at 3 days and 1 week post heat-shock in Crei\I-CreI^hs.PR Crei\I-CreI^hs.PR/+ mutants but is much decreased in Crei\I-CreI^hs.PR Crei\I-CreI^hs.PR/Crei\I-CreI^hs.PR mutants at the later time-point, as compared to Crei\I-CreI^hs.PR-only flies.

mei-41^D3;Crei\I-CreI^hs.PR as well as Scer\GAL4^nos.PU>tefu^HMS02790;Crei\I-CreI^hs.PR double mutants have fewer GSCs per germarium, whereas lok^KD/+;Crei\I-CreI^hs.PR, lok^KD/lok^KD;Crei\I-CreI^hs.PR or lok^KD/lok^p6;Crei\I-CreI^hs.PR mutants have more GSCs than wild-type controls even without the induction of DNA damage by heat-shock.

Crei\I-CreI^hs.PR germaria in which DNA damage has been evoked by heat-shock accumulate cystoblast-like single cells and this accumulation is accelerated by lok^p6 or lok^KD heterozygosity (significantly increased number of CB-like cells observed already at 3 days post heat-shock) and is strongly enhanced by bam^Δ86 heterozygosity. By contrast, both lok^p6 or lok^KD homo- or transheterozygosity completely abolishes the CB-like cell accumulation phenotype.

(Ma et al., 2016)

BubR1^unspecified, Crei\I-CreI^hs.PR mutants exhibit a drop in anaphase cells essentially the same as seen in Crei\I-CreI^hs.PR.

(Royou et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6936

P{hs-I-CreI.R}2A, v¹; ry⁵⁰⁶

6937

w¹¹¹⁸; P{hs-I-CreI.R}1A Sb¹/TM6

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Crei\I-CreI^hs.PR

Name Synonyms

heat shock construct of Rong

(Rong et al., 2002)

Secondary FlyBase IDs

References (9)

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