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General Information
Symbol
Crei\I-CreIhs.PR
Species
C. reinhardtii
Name
heat shock construct of Rong
FlyBase ID
FBal0137561
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hs-I-CreI
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An Hsp70 promoter drives expression of Crei\I-CreI.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heat-shock induced expression of Crei\I-CreIhs.PR (to introduce DNA damage) in adult female flies leads to cell cycle arrest in the germline stem cells (GSCs) directly after DNA damage and then the cells resume rapid proliferation. Following the heat-shock, the mutant germaria display a reduced number of GSCs relative to no heat-shock controls and this number stays low even a week after the damage induction. No apoptotic GSCs (visualized by *Casp3 labelling) are detected in the challenged mutant germaria and the size of the nucleolus in the GSCs (indicative of rRNA production) is comparable to controls.

Crei\I-CreIhs.PR germaria in which DNA damage has been evoked by heat-shock accumulate cystoblast-like single cells compared to undamaged controls.

Expression of Crei\I-CreIhs.PR using heat shock results in a dramatic drop in the mitotic index of larval brain neuroblasts. Expression of Crei\I-CreIhs.PR using heat shock results in recombination between the X and Y chromosomes in the heterochromatic regions where the rDNA is located; approximately 20% of the offspring of males expressing Crei\I-CreIhs.PR show exceptional inheritance of X- and Y-linked markers, consistent with exchange at rDNA loci.

Heat shock induction of Crei\I-CreIhs.PR produces a high frequency of abnormal anaphases, in which chromosome fagments remain at the metaphase plate. Besides an overall reduction in the mitotic index, the frequency of anaphase cells drops significantly compared to control cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, enhanceable by Scer\GAL4nos.PU/mei-41HMS02331

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, enhanceable by Scer\GAL4nos.PU/tefuHMS02790

Crei\I-CreIhs.PR has increased cell number | female | adult stage | temperature conditional phenotype, enhanceable by lok[+]/lokKD

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, enhanceable by p535A-1-4/p535A-1-4

Crei\I-CreIhs.PR has increased cell number | female | adult stage | temperature conditional phenotype, enhanceable by bam[+]/bamΔ86

Crei\I-CreIhs.PR has increased cell number | female | adult stage | temperature conditional phenotype, enhanceable by lok[+]/lokp6

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, enhanceable by mei-41[+]/mei-41D3

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, enhanceable by mei-41D3/mei-41D3

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, enhanceable by mei-41GL00284/Scer\GAL4nos.PU

NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lok[+]/lokKD

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lokKD/lokKD

Crei\I-CreIhs.PR has increased cell number | female | adult stage | temperature conditional phenotype, suppressible by lokKD/lokKD

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lokp6/lokKD

Crei\I-CreIhs.PR has increased cell number | female | adult stage | temperature conditional phenotype, suppressible by lokp6/lokKD

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by p535A-1-4/p53[+]

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by tkvQ253D.UASp/Scer\GAL4nos.PU

Crei\I-CreIhs.PR has decreased cell number | adult stage | temperature conditional phenotype, suppressible by lok[+]/lokp6

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lokp6/lokp6

Crei\I-CreIhs.PR has increased cell number | female | adult stage | temperature conditional phenotype, suppressible by lokp6/lokp6

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, suppressible by lokGL00020/Scer\GAL4nos.PU

NOT suppressed by
Statement
Reference

Crei\I-CreIhs.PR has decreased cell number | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4nos.PU/BacA\p35UASp.Tag:FLAG

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4nos.PU/grpHMS01573

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, non-suppressible by grpHMC05162/Scer\GAL4nos.PU

Crei\I-CreIhs.PR has decreased cell number | female | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4nos.PU/shgUASp.Tag:FLAG

Enhancer of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by Scer\GAL4nos.PU/mei-41HMS02331

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by Scer\GAL4nos.PU/tefuHMS02790

Crei\I-CreIhs.PR has cystoblast | supernumerary | adult stage | temperature conditional phenotype, enhanceable by lok[+]/lokKD

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by p535A-1-4/p535A-1-4

Crei\I-CreIhs.PR has cystoblast | supernumerary | adult stage | temperature conditional phenotype, enhanceable by bam[+]/bamΔ86

Crei\I-CreIhs.PR has cystoblast | supernumerary | adult stage | temperature conditional phenotype, enhanceable by lok[+]/lokp6

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by mei-41[+]/mei-41D3

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by mei-41D3/mei-41D3

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, enhanceable by mei-41GL00284/Scer\GAL4nos.PU

Suppressed by
Statement
Reference

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok[+]/lokKD

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lokKD/lokKD

Crei\I-CreIhs.PR has cystoblast | supernumerary | adult stage | temperature conditional phenotype, suppressible by lokKD/lokKD

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lok[+]/lokp6

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lokp6/lokKD

Crei\I-CreIhs.PR has cystoblast | supernumerary | adult stage | temperature conditional phenotype, suppressible by lokp6/lokKD

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by p535A-1-4/p53[+]

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by tkvQ253D.UASp/Scer\GAL4nos.PU

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lokp6/lokp6

Crei\I-CreIhs.PR has cystoblast | supernumerary | adult stage | temperature conditional phenotype, suppressible by lokp6/lokp6

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, suppressible by lokGL00020/Scer\GAL4nos.PU

NOT suppressed by
Statement
Reference

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4nos.PU/BacA\p35UASp.Tag:FLAG

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4nos.PU/grpHMS01573

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by grpHMC05162/Scer\GAL4nos.PU

Crei\I-CreIhs.PR has female germline stem cell | adult stage | temperature conditional phenotype, non-suppressible by Scer\GAL4nos.PU/shgUASp.Tag:FLAG

Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The loss of germline stem cells (GSCs) in female germaria observed upon heat-shock-induced expression of Crei\I-CreIhs.PR (to introduce DNA damage) cannot be rescued by Scer\GAL4nos.PU-driven expression of BacA\p35UASp.Tag:FLAG in the mutant background.

The damage-evoked loss of GSCs upon expression of Crei\I-CreIhs.PR (induced by heat-shock to introduce DNA breaks) is rescued by combination with lokp6 or lokKD in hetero-, homo- or transheterozygosity as these double mutant flies contain significantly higher number of GSCs both at 3 days and 1 week post heat-shock compared to Crei\I-CreIhs.PR single mutants. Similarly, Scer\GAL4nos.PU-driven expression of lokGL00020 or tkvQ253D.Scer\UAS.P\T or simultaneous expression of both tkvQ253D.Scer\UAS.P\T and either saxQ263D.UASp or shgUASp.Tag:FLAG also significantly suppresses the damage-evoked GSC loss in Crei\I-CreIhs.PR flies. In contrast, this loss is further exacerbated by mei-41D3 in homo- or heterozygosity or by expression of mei-41GL00284, mei-41HMS02331 or tefuHMS02790 under the Scer\GAL4nos.PU driver, expression of either grpHMS01573 or grpHMC05162 or shgUASp.Tag:FLAG has no effect on the GSCs number in Crei\I-CreIhs.PR mutant background either in the presence or absence of DNA damage. The loss of GSCs in damaged Crei\I-CreIhs.PR germaria is slightly alleviated by combination with a single copy of p535A-1-4 but is actually made worse by p535A-1-4 homozygosity.

The number of GSCs remaining after DNA-damage has been induced is slightly higher both at 3 days and 1 week post heat-shock in Crei\I-CreIhs.PR Crei\I-CreIhs.PR/+ mutants but is much decreased in Crei\I-CreIhs.PR Crei\I-CreIhs.PR/Crei\I-CreIhs.PR mutants at the later time-point, as compared to Crei\I-CreIhs.PR-only flies.

mei-41D3;Crei\I-CreIhs.PR as well as Scer\GAL4nos.PU>tefuHMS02790;Crei\I-CreIhs.PR double mutants have fewer GSCs per germarium, whereas lokKD/+;Crei\I-CreIhs.PR, lokKD/lokKD;Crei\I-CreIhs.PR or lokKD/lokp6;Crei\I-CreIhs.PR mutants have more GSCs than wild-type controls even without the induction of DNA damage by heat-shock.

Crei\I-CreIhs.PR germaria in which DNA damage has been evoked by heat-shock accumulate cystoblast-like single cells and this accumulation is accelerated by lokp6 or lokKD heterozygosity (significantly increased number of CB-like cells observed already at 3 days post heat-shock) and is strongly enhanced by bamΔ86 heterozygosity. By contrast, both lokp6 or lokKD homo- or transheterozygosity completely abolishes the CB-like cell accumulation phenotype.

BubR1unspecified, Crei\I-CreIhs.PR mutants exhibit a drop in anaphase cells essentially the same as seen in Crei\I-CreIhs.PR.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Crei\I-CreIhs.PR
Name Synonyms
heat shock construct of Rong
Secondary FlyBase IDs
    References (5)