The maternal expression of tkvQ253D.Scer\UAS.P\T, either under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 or in the absence of the Gal4 driver, partially rescues the lethality of the progeny from dpphr27/+ fathers and lolal1122/+ mothers and fully rescues the near lethality of the progeny from dpphr4/+ fathers and lolal1122/+ mothers; this maternal expression also fully rescues the embryo ventralization of the progeny from dpphr4/+ fathers and lolal1122/+ mothers if under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, but only partially in the absence of the Gal4 driver.
bamhs.PO + daily heat shock daily starting 7 days after egg laying significantly rescues the tumorous ovariole phenotype of tkvQ253D.Scer\UAS.P\T; Scer\GAL4nos.UTR.T:Hsim\VP16 females: 3 or 4 days after eclosion all ovarioles contain egg chambers with large, polyploid nuclei and marker expression consistent with differentiation. Although many of these egg chambers have a normal 15:1 nurse cell to oocyte ratio, some contain an abnormal number of germ cells. 10 days after eclosion followed by daily heat shock, the females have ovarioles with completely normal morphology. The germaria in these females appear completely normal apart from a number which are devoid of germline stem cells. Many of these females lay eggs with normal morphology. However, the resulting embryos lack cuticle.
The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreIhs.PR (to introduce DNA damage) is suppressed by Scer\GAL4nos.PU-driven expression of tkvQ253D.Scer\UAS.P\T or simultaneous expression of both tkvQ253D.Scer\UAS.P\T and either saxQ263D.UASp or shgUASp.Tag:FLAG.