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General Information
Symbol
Dmel\tkvQ253D.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0177448
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of mutated tkv from tkvQ253D.SP6 is under the control of UASp regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Germ-line expression of tkvQ253D.Scer\UAS.P\T using Scer\GAL4nos.UTR.T:Hsim\VP16 induces a tumorous phenotype (all germline stem cell-like cells, no cysts).

1-3 day old tkvQ253D.Scer\UAS.P\T; Scer\GAL4nos.UTR.T:Hsim\VP16 females have `tumorous' ovarioles filled with cells whose marker expression and morphology are consistent with being germline stem cells, including the presence of spectrosomes. These ovarioles do not contain any cystoblasts or differentiating egg chambers. Many of these ectopic germline stem cells are in the process of dividing. Expression of some germ cell markers declines in older females of this genotype (>5 days old), and some of the ectopic germline stem cells become apoptotic.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The maternal expression of tkvQ253D.Scer\UAS.P\T, either under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 or in the absence of the Gal4 driver, partially rescues the lethality of the progeny from dpphr27/+ fathers and lolal1122/+ mothers and fully rescues the near lethality of the progeny from dpphr4/+ fathers and lolal1122/+ mothers; this maternal expression also fully rescues the embryo ventralization of the progeny from dpphr4/+ fathers and lolal1122/+ mothers if under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, but only partially in the absence of the Gal4 driver.

bamhs.PO + daily heat shock daily starting 7 days after egg laying significantly rescues the tumorous ovariole phenotype of tkvQ253D.Scer\UAS.P\T; Scer\GAL4nos.UTR.T:Hsim\VP16 females: 3 or 4 days after eclosion all ovarioles contain egg chambers with large, polyploid nuclei and marker expression consistent with differentiation. Although many of these egg chambers have a normal 15:1 nurse cell to oocyte ratio, some contain an abnormal number of germ cells. 10 days after eclosion followed by daily heat shock, the females have ovarioles with completely normal morphology. The germaria in these females appear completely normal apart from a number which are devoid of germline stem cells. Many of these females lay eggs with normal morphology. However, the resulting embryos lack cuticle.

Xenogenetic Interactions
Statement
Reference

The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreIhs.PR (to introduce DNA damage) is suppressed by Scer\GAL4nos.PU-driven expression of tkvQ253D.Scer\UAS.P\T or simultaneous expression of both tkvQ253D.Scer\UAS.P\T and either saxQ263D.UASp or shgUASp.Tag:FLAG.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
tkvQ253D.Scer\UAS.P\T
tkvQ253D.UASp
Name Synonyms
Secondary FlyBase IDs
    References (5)