FlyBase Allele Report: Dmel\tkv[Q253D.UASp]

General Information

Symbol

Dmel\tkv^Q253D.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0177448

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UASp-tkv.Q253D}

Also Known As

UASp-Tkv^Act, UASp-Tkv^QD

Key Links

Transgenic product class

missense_variant

(Casanueva and Ferguson, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Casanueva and Ferguson, 2004)

Mutagen

in vitro construct

(Casanueva and Ferguson, 2004)

Progenitor genotype

Carried in construct

P{UASp-tkv.Q253D}

Cytology

Description

Expression of mutated tkv from tkv^Q253D.SP6 is under the control of UASp regulatory sequences.

(Casanueva and Ferguson, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

tkv

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis, with Scer\GAL4^nanos.PG

(Azlan et al., 2025)

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

(Casanueva and Ferguson, 2004)

hyperplasia | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

Germ-line expression of tkv^{Q253D.Scer\UAS.P\T} using Scer\GAL4^{nos.UTR.T:Hsim\VP16} induces a tumorous phenotype (all germline stem cell-like cells, no cysts).

(Dolezal et al., 2015)

1-3 day old tkv^{Q253D.Scer\UAS.P\T}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} females have `tumorous' ovarioles filled with cells whose marker expression and morphology are consistent with being germline stem cells, including the presence of spectrosomes. These ovarioles do not contain any cystoblasts or differentiating egg chambers. Many of these ectopic germline stem cells are in the process of dividing. Expression of some germ cell markers declines in older females of this genotype (>5 days old), and some of the ectopic germline stem cells become apoptotic.

(Casanueva and Ferguson, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tkv^Q253D.UASp/Scer\GAL4^nanos.PG is an enhancer of abnormal cell number | oogenesis phenotype of Scer\GAL4^nanos.PG, bbn^KK111109

(Azlan et al., 2025)

Suppressor of

Statement

Reference

tkv^Q253D.UASp/Scer\GAL4^nanos.PU is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

tkv^Q253D.UASp, sax^Q263D.UASp, Scer\GAL4^nanos.PU is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

tkv^Q253D.UASp, Scer\GAL4^nanos.PU, shg^{UASp.Tag:FLAG} is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

tkv^Q253D.UASp/Scer\GAL4^{VP16.nanos.UTR} is a suppressor | partially of lethal | maternal effect phenotype of dpp^hr27, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

tkv^Q253D.UASp is a suppressor | partially of lethal | maternal effect phenotype of dpp^hr27, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

tkv^Q253D.UASp/Scer\GAL4^{VP16.nanos.UTR} is a suppressor of partially lethal - majority die | maternal effect phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

tkv^Q253D.UASp is a suppressor of partially lethal - majority die | maternal effect phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp has germarium phenotype, suppressible | partially by lili²/lili[+]

(Dolezal et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp has female germline stem cell | increased number phenotype, suppressible | partially by lili²/lili[+]

(Dolezal et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp has cystoblast phenotype, suppressible by bam^hs.PO

(Casanueva and Ferguson, 2004)

Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp has egg chamber phenotype, suppressible by bam^hs.PO

(Casanueva and Ferguson, 2004)

Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp has ovary phenotype, suppressible by bam^hs.PO

(Casanueva and Ferguson, 2004)

Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp has female germline stem cell | ectopic phenotype, suppressible by bam^hs.PO

(Casanueva and Ferguson, 2004)

Suppressor of

Statement

Reference

tkv^Q253D.UASp/Scer\GAL4^nanos.PU is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

tkv^Q253D.UASp, sax^Q263D.UASp, Scer\GAL4^nanos.PU is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

tkv^Q253D.UASp, Scer\GAL4^nanos.PU, shg^{UASp.Tag:FLAG} is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

tkv^Q253D.UASp is a suppressor | partially of embryo | maternal effect phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

tkv^Q253D.UASp/Scer\GAL4^{VP16.nanos.UTR} is a suppressor of embryo | maternal effect phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

NOT Suppressor of

Statement

Reference

tkv^Q253D.UASp/Scer\GAL4^nanos.PG is a non-suppressor of ovariole phenotype of Scer\GAL4^nanos.PG, bbn^KK111109

(Azlan et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

The maternal expression of tkv^{Q253D.Scer\UAS.P\T}, either under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} or in the absence of the Gal4 driver, partially rescues the lethality of the progeny from dpp^hr27/+ fathers and lolal¹¹²²/+ mothers and fully rescues the near lethality of the progeny from dpp^hr4/+ fathers and lolal¹¹²²/+ mothers; this maternal expression also fully rescues the embryo ventralization of the progeny from dpp^hr4/+ fathers and lolal¹¹²²/+ mothers if under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, but only partially in the absence of the Gal4 driver.

(Quijano et al., 2016)

bam^hs.PO + daily heat shock daily starting 7 days after egg laying significantly rescues the tumorous ovariole phenotype of tkv^{Q253D.Scer\UAS.P\T}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} females: 3 or 4 days after eclosion all ovarioles contain egg chambers with large, polyploid nuclei and marker expression consistent with differentiation. Although many of these egg chambers have a normal 15:1 nurse cell to oocyte ratio, some contain an abnormal number of germ cells. 10 days after eclosion followed by daily heat shock, the females have ovarioles with completely normal morphology. The germaria in these females appear completely normal apart from a number which are devoid of germline stem cells. Many of these females lay eggs with normal morphology. However, the resulting embryos lack cuticle.

(Casanueva and Ferguson, 2004)

Xenogenetic Interactions

Statement

Reference

The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreI^hs.PR (to introduce DNA damage) is suppressed by Scer\GAL4^nos.PU-driven expression of tkv^{Q253D.Scer\UAS.P\T} or simultaneous expression of both tkv^{Q253D.Scer\UAS.P\T} and either sax^Q263D.UASp or shg^{UASp.Tag:FLAG}.

(Ma et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

604934

y¹ w^*; P{UASp-tkv.Q253D}3/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tkv*

(Ma et al., 2016)

tkv^{Q253D.Scer\UAS.P\T}

tkv^Q253D.UASp

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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