FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\shgUASp.Tag:FLAG
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General Information
Symbol
Dmel\shgUASp.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0301426
Feature type
allele
Associated gene
Dmel\shg
Associated Insertion(s)
Carried in Construct
P{UASp-shg.FLAG}
Also Known As
UASp-shg
Key Links
Transgenic product class
wild_type
(Pan et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Pan et al., 2007)
Mutagen
in vitro construct
(Pan et al., 2007)
Progenitor genotype
Carried in construct
P{UASp-shg.FLAG}
Cytology
Description

UASp regulatory sequences drive expression of the coding region of shg tagged with Tag:FLAG.

(Pan et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
shg
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Germline stem cell (GSC) clones expressing shgScer\UAS.P\T.T:Zzzz\FLAG via Scer\GAL4nos.UTR.T:Hsim\VP16 (using a FLP-FRT method) show greater contact with the germarium cap cells than control clones, which leads the mutant cells to out-compete wild type cells in the niche.

      (Jin et al., 2008)

      7-day old germaria expressing shgScer\UAS.P\T.T:Zzzz\FLAG via Scer\GAL4nos.PU have normal numbers of germline cell stems (GSCs) and cap cells. However, 63-day old germaria have a significantly greater number of GSCs and cap cells compared to controls, demonstrating increased maintenance rates. They also carry significantly more cysts than controls at this age.

      (Pan et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      tkvQ253D.UASp, Scer\GAL4nanos.PU, shgUASp.Tag:FLAG is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4nanos.PU/shgUASp.Tag:FLAG is a non-suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      tkvQ253D.UASp, Scer\GAL4nanos.PU, shgUASp.Tag:FLAG is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4nanos.PU/shgUASp.Tag:FLAG is a non-suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

      (Ma et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreIhs.PR (to introduce DNA damage) is suppressed by Scer\GAL4nos.PU-driven simultaneous expression of both tkvQ253D.Scer\UAS.P\T and shgUASp.Tag:FLAG. Expression of shgUASp.Tag:FLAG alone has no effect on the GSCs number in Crei\I-CreIhs.PR mutant background either in the presence or absence of DNA damage.

      (Ma et al., 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      shgScer\UAS.P\T.T:Zzzz\FLAG
      shgUASp.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (4)