FlyBase Allele Report: Dmel\Dis3l2[SK5]

General Information

Symbol

Dmel\Dis3l2^SK5

Species

D. melanogaster

Name

FlyBase ID

FBal0326311

Feature type

allele

Associated gene

Dmel\Dis3l2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Lin et al., 2017)

Mutagen

CRISPR/Cas9

(Lin et al., 2017)

Progenitor genotype

Cytology

Description

4bp deletion within the Dis3l2 coding sequence.

(Lin et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:213,428..213,431 [+]

Comment:

4bp deletion within the Dis3l2 coding sequence.

(Lin et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female semi-sterile (with Df(3L)ED201)

(Lin et al., 2017)

male sterile (with Df(3L)ED201)

(Lin et al., 2017)

semi-sterile

(Lin et al., 2017)

viable

(Lin et al., 2017)

Phenotype Manifest In

cystic bulge

(Lin et al., 2017)

cystic bulge (with Df(3L)ED201)

(Lin et al., 2017)

individualization stage spermatid

(Lin et al., 2017)

individualization stage spermatid (with Df(3L)ED201)

(Lin et al., 2017)

seminal vesicle

(Lin et al., 2017)

seminal vesicle (with Df(3L)ED201)

(Lin et al., 2017)

sperm individualization complex

(Lin et al., 2017)

sperm individualization complex (with Df(3L)ED201)

(Lin et al., 2017)

waste bag

(Lin et al., 2017)

waste bag (with Df(3L)ED201)

(Lin et al., 2017)

Detailed Description

Statement

Reference

The fertility of both male and female Dis3l2^SK5 homozygotes as well as Dis3l2^SK5/Df(3L)ED201 female transheterozygotes is strongly decreased, Dis3l2^SK5/Df(3L)ED201 males are completely sterile.

The germline stem cell (GSCs) niche in the Dis3l2^SK5/Df(3L)ED201 testes is not apparently perturbed and the number of GSCs contacting the somatic hub is similar to wild-type controls but the spermatid differentiation is impaired: the spermatid nuclei are poorly bundled in the basal region and the individualization process is flawed - no cystic bulges (CBs) are formed and the randomly moving individualization complexes do not form any waste bags (WBs) either. Similar phenotype (no CBs no waste bags) is observed in Dis3l2^SK5 homozygotes and both Dis3l2^SK5/Df(3L)ED201 and Dis3l2^SK5/Dis3l2^SK5 mutants show rudimentary seminal vesicle devoid of mature sperm.

(Lin et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dis3l2^SK5 has semi-sterile phenotype, enhanceable by Tailor^c4-1/Tailor^f05717

(Lin et al., 2017)

Dis3l2^SK5 has semi-sterile phenotype, enhanceable by Tailor^f05717/Tailor^c4-6

(Lin et al., 2017)

Enhancer of

Statement

Reference

Dis3l2^SK5/Dis3l2^SK5 is an enhancer of semi-fertile phenotype of Tailor^c4-1/Tailor^f05717

(Lin et al., 2017)

Dis3l2^SK5/Dis3l2^SK5 is an enhancer of semi-fertile phenotype of Tailor^f05717/Tailor^c4-6

(Lin et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dis3l2^SK5/Dis3l2^SK5 is an enhancer of individualization stage spermatid phenotype of Tailor^f05717/Tailor^c4-6

(Lin et al., 2017)

Dis3l2^SK5/Dis3l2^SK5 is an enhancer of sperm individualization complex phenotype of Tailor^f05717/Tailor^c4-6

(Lin et al., 2017)

Dis3l2^SK5/Dis3l2^SK5 is an enhancer of cystic bulge phenotype of Tailor^f05717/Tailor^c4-6

(Lin et al., 2017)

Dis3l2^SK5/Dis3l2^SK5 is an enhancer of seminal vesicle phenotype of Tailor^f05717/Tailor^c4-6

(Lin et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The subfertility of Tailor^f05717/Tailor^c4-1 or Tailor^f05717/Tailor^c4-6 mutants and of the Dis3l2^SK5 homozygous mutants is exacerbated further in double mutants and the Tailor^f05717/Tailor^c4-1;Dis3l2^SK5/Dis3l2^SK5 or Tailor^f05717/Tailor^c4-6;Dis3l2^SK5/Dis3l2^SK5 double mutants are completely sterile.

The spermatid differentiation defects observed in testes of Tailor^f05717/Tailor^c4-6 mutant males are enhanced by combination with two copies of Dis3l2^SK5 : Like in the Dis3l2^SK5 single mutants, no cystic bulges or waste bags are formed and the double mutants bear only rudimentary seminal vesicles devoid of mature sperm.

(Lin et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dis3L2[SK5]

(Lin et al., 2017)

Dis3l2^SK5

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close