FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Arf1T31N.UAS.cWa
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General Information
Symbol
Dmel\Arf1T31N.UAS.cWa
Species
D. melanogaster
Name
FlyBase ID
FBal0326762
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UAS regulatory sequences drive expression of a GDP-locked form of Arf79F (carries the amino acid replacement T31N).

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Arf79FT31N.Scer\UAS.cWa under the control of Scer\GAL4ppk.PU leads to dorsal multidendritic ddaC neurons exhibiting a significantly simplified dendritic tree at the white pupa stage and a small but significant delay in dendrite pruning at 16h-18h after puparium formation, as compared to controls.

Clonal expression under the control of Scer\GAL4109(2)80 leads to dorsal multidendritic ddaD/E neurons exhibiting a simplified dendritic tree at the white pupa stage and exhibiting defective dendrite pruning, as shown by the retention of some dendrite branches at 19h after puparium formation, as compared to controls.

Expression under the combined control of Scer\GAL4GSG2295 and RU486 treatment from mid-third instar larval stage onwards leads to dorsal multidendritic ddaC neurons exhibiting a fully penetrant pruning defect, as shown by the persistence some dendritic branches at 16h-18h after puparium formation, but exhibiting no obvious defects in the dendritic tree at the white pupa stage, and leads to no defects in the elimination of ddaF neurons by apoptosis at 16h after puparium formation, as compared to controls; at the wandering third instar larval stage, this expression also leads to dorsal multidendritic ddaC neurons exhibiting Golgi apparatus defects, as shown by severe decreases in the numbers of GalT or GM130 punctate structures in the soma, exhibiting decreased protein secretion, but exhibiting no obvious defects in ER exit sites, as shown by the apparently normal pattern of Sec31 punctate structures in the soma, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Arf1T31N.UAS.cWa
Arf79FT31N.Scer\UAS.cWa
Arf79FT31N.UAS.cWa
Name Synonyms
Secondary FlyBase IDs
    References (4)