FlyBase Allele Report: Dmel\mir-31a[sponge.UAS.EGFP]

General Information

Symbol

Dmel\mir-31a^{sponge.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0326960

Feature type

allele

Associated gene

Dmel\mir-31a

Associated Insertion(s)

Carried in Construct

P{UAS-EGFP.mir-31a.sponge}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Foo et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-EGFP.mir-31a.sponge}

Cytology

Description

UAS regulates expression of ten copies of the reverse complement of the mature mir-31a 5p sequence with a point mutation made in the tenth position to change a C to an A, downstream of EGFP.

(Foo et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-31a

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^elav.PU

(Foo et al., 2017)

decreased cell number | adult stage, with Scer\GAL4^insc-Mz1407

(Foo et al., 2017)

decreased cell number | adult stage, with Scer\GAL4^mir-31a, mir-31a^KO.Gal4

(Foo et al., 2017)

decreased cell number | adult stage, with Scer\GAL4^wor.PA

(Foo et al., 2017)

Phenotype Manifest In

adult CNS glial cell, with Scer\GAL4^elav.PU

(Foo et al., 2017)

adult CNS glial cell, with Scer\GAL4^insc-Mz1407

(Foo et al., 2017)

adult CNS glial cell, with Scer\GAL4^mir-31a

(Foo et al., 2017)

adult CNS glial cell, with Scer\GAL4^wor.PA

(Foo et al., 2017)

Detailed Description

Statement

Reference

Adulthood-only expression (by the use of Gal80[ts]) of mir-31a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^insc-Mz1407 or Scer\GAL4^mir-31a (the intrinsic Gal4 driver of mir-31a^KO.Gal4) or 'continual' expression of mir-31a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav.PU, Scer\GAL4^insc-Mz1407 or Scer\GAL4^wor.PA, but not Scer\GAL4^repo.PU or Scer\GAL4^nSyb.PU, leads to a significant decrease in the number of adult brain glial cells at day 7 post eclosion, as compared to controls.

(Foo et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, mir-31a^{sponge.UAS.EGFP} has decreased cell number | adult stage phenotype, suppressible by Df(3L)H99/+

(Foo et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, mir-31a^{sponge.UAS.EGFP} has adult CNS glial cell phenotype, suppressible by Df(3L)H99/+

(Foo et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of brain glial cells observed in 7 days old adults expressing mir-31a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^insc-Mz1407 is suppressed by heterozygosity for Df(3L)H99.

(Foo et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76033

w¹¹¹⁸; P{UAS-EGFP.mir-31a.sponge}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-31a^{sponge.Scer\UAS.T:Avic\GFP-EGFP}

mir-31a^{sponge.UAS.EGFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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