FlyBase Allele Report: Dmel\mir-31a[KO.Gal4]

General Information

Symbol

Dmel\mir-31a^KO.Gal4

Species

D. melanogaster

Name

FlyBase ID

FBal0326959

Feature type

allele

Associated gene

Dmel\mir-31a

Associated Insertion(s)

TI{GAL4}mir-31a^KO.Gal4

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

recombination

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Foo et al., 2017)

Mutagen

recombination

(Foo et al., 2017)

Progenitor genotype

TI{TI}mir-31a^KO.RMCE

(Foo et al., 2017)

Associated Insertion(s)

TI{GAL4}mir-31a^KO.Gal4

Cytology

Description

The mini-white cassette of the progenitor (TI{TI}mir-31a^KO.RMCE) has been replaced with sequence encoding a GAL4 driver.

(Foo et al., 2017)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^mir-31a, mir-31a^{sponge.UAS.EGFP}

(Foo et al., 2017)

decreased cell number | adult stage (with mir-31a^KO)

(Foo et al., 2017)

decreased cell number | adult stage | recessive

(Foo et al., 2017)

Phenotype Manifest In

adult CNS glial cell

(Foo et al., 2017)

adult CNS glial cell (with mir-31a^KO)

(Foo et al., 2017)

Detailed Description

Statement

Reference

At day 7 post eclosion, mir-31a^KO.Gal4/mir-31a^KO transheterozygotes, mir-31a^KO.Gal4 homozygotes, and mir-31a^KO.Gal4 heterozygotes also expressing mir-31a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^mir-31a (the intrinsic Gal4 driver of mir-31a^KO.Gal4), but not mir-31a^KO.Gal4 heterozygotes, exhibit a decrease in the number of adult brain glial cells, as compared to controls.

(Foo et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-31a^KO/mir-31a^KO.Gal4 has decreased cell number | adult stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Rchy1^HMJ22014

(Foo et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

mir-31a^KO/mir-31a^KO.Gal4 has adult CNS glial cell phenotype, suppressible by Scer\GAL4^mir-31a/Rchy1^HMJ22014

(Foo et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of adult brain glial cells in 7 days old mir-31a^KO.Gal4/mir-31a^KO transheterozygotes is suppressed by the expression of Rchy1^HMJ22014 under the control of Scer\GAL4^mir-31a (the intrinsic Gal4 driver of mir-31a^KO.Gal4).

(Foo et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mir-31a^KO.Gal4 is rescued by Scer\GAL4^mir-31a/mir-31a^UAS.cSb

(Foo et al., 2017)

Comments

The decreased number of 7 days old adult brain glial cells present in mir-31a^KO.Gal4 homozygotes is rescued by the expression of mir-31a^Scer\UAS.cSb under the control of Scer\GAL4^mir-31a (the intrinsic Gal4 driver of mir-31a^KO.Gal4).

(Foo et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76034

w¹¹¹⁸; TI{GAL4}mir-31a^KO.Gal4

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

miR-31a-Gal4

(Foo et al., 2017)

mir-31a^KO.Gal4

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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