FlyBase Allele Report: Dmel\mir-31a[KO]

General Information

Symbol

Dmel\mir-31a^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0301924

Feature type

allele

Associated gene

Dmel\mir-31a

Associated Insertion(s)

TI{TI}mir-31a^KO

Carried in Construct

Also Known As

mir-31a KO

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)

Mutagen

ends-out gene targeting

(Chen et al., 2014)

FLPase

(Chen et al., 2014)

I-SceI

(Chen et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-31a^KO

Cytology

Description

The mir-31a hairpin has been deleted and replaced by a loxP cassette containing the w^+mW.hs marker.

(Chen et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

mir-31a^KO homozygotes do not exhibit obvious defects in life-span, as compared to controls; their adult brains exhibit transient decreases in the numbers of either total glial cells or astrocytes, but not of cortex glial cells or of ensheathing glial cells, at days 7 and 21, but not at day 2 post eclosion, which is associated with a moderate increase in the proportion of apoptotic glial cells at day 7 post eclosion, as compared to controls; at 7 days post eclosion, their adult brains also show a small but significant decrease in the number of neurons and significant increases in the proportions of either total cells or glial lineage cells undergoing DNA replication, as compared to controls; in lineage-tracing experiments, their adult neuroglia progenitor cells give rise to a significantly increased proportion of glial cells, but not of neurons, as compared to controls.

7 days old mir-31a^KO/mir-31a^KO.Gal4 transheterozygotes exhibit a significant decrease in the number of adult brain glial cells, as compared to controls.

(Foo et al., 2017)

Brains of mir-31a^KO homozygous mutant adults display loss of glial cells over time and at day 7 post-eclosion their number is significantly reduced compared to controls (although the mutant adults emerge with comparable number of brain glia).

(Foo, 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-31a^KO has decreased cell number | adult stage phenotype, suppressible by Df(3L)H99/+

(Foo et al., 2017)

mir-31a^KO has decreased cell number | adult stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Rchy1^HMJ22014

(Foo et al., 2017)

mir-31a^KO/mir-31a^KO.Gal4 has decreased cell number | adult stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Rchy1^HMJ22014

(Foo et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

mir-31a^KO has adult CNS glial cell phenotype, suppressible by Df(3L)H99/+

(Foo et al., 2017)

mir-31a^KO has adult CNS glial cell phenotype, suppressible by Scer\GAL4^insc-Mz1407/Rchy1^HMJ22014

(Foo et al., 2017)

mir-31a^KO/mir-31a^KO.Gal4 has adult CNS glial cell phenotype, suppressible by Scer\GAL4^mir-31a/Rchy1^HMJ22014

(Foo et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of adult brain glial cells in 7 days old mir-31a^KO homozygotes is suppressed either by heterozygosity for Df(3L)H99 or by the expression of Rchy1^HMJ22014 under the control of Scer\GAL4^insc-Mz1407; the decreased number of adult brain glial cells in 7 days old mir-31a^KO/mir-31a^KO.Gal4 transheterozygotes is suppressed by the expression of Rchy1^HMJ22014 under the control of Scer\GAL4^mir-31a (the intrinsic Gal4 driver of mir-31a^KO.Gal4).

(Foo et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mir-31a^KO is rescued by Scer\GAL4^insc-Mz1407/mir-31a^UAS.cSb

(Foo et al., 2017)

Comments

The decreased number of adult brain glial cells present in 7 days old mir-31a^KO homozygotes is rescued by the expression of mir-31a^Scer\UAS.cSb under the control of Scer\GAL4^insc-Mz1407.

(Foo et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58928

w^*; TI{TI}mir-31a^KO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

miR-31a KO

(Iftikhar et al., 2019)

miR-31a^KO

(Foo et al., 2017)

mir-31a KO

(Foo, 2017)

mir-31a-KO

(Sreejith et al., 2025)

mir-31a^KO

(Niu et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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